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Help analyzing fastq file!



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  • Help analyzing fastq file!

    Hi everyone, I am new to this forum and joined to get some help with analyzing a fastq file from study Ruminomics - 1000 cows microbiome amplicon survey

    I am downloading raw fastq file to analyze with QIIME2 but I am unable to track information such as barcodes etc since this is the first time I am using someone else data for practicing. The header of fastq files looks like this:

    @HWI-D00104:273:C6EDRANXX:8:1101:3922:3491_CONS_SUB_SUB_CMP ali_length=104; seq_ab_match=100; tail_quality=29.1; reverse_match=cctacggctaccttgttac; seq_a_deletion=0; sample=IT646; SGL_LAB=RS; WP=3; forward_match=cctgctccttgcacacac; forward_primer=cctgctccttgcacacac; reverse_primer=cctacggctaccttgttac; EarTag=IT098990178892; forward_score=72.0; score=302.232778128; seq_a_mismatch=4; forward_tag=tgctccaa; seq_b_mismatch=0; experiment=Plate11_ArchA_R1; mid_quality=51.2936507937; avg_quality=48.5410958904; seq_a_single=21; score_norm=2.90608440508; Azienda=Bianchini; reverse_score=76.0; direction=reverse; seq_b_insertion=0; seq_b_deletion=0; SamplingDate=18.12.2014; seq_a_insertion=0; seq_length_ori=146; reverse_tag=tgctccaa; goodAli=Alignement; AnimalID=4; seq_length=87; N_LAB=282; status=full; mode=alignment; head_quality=33.3; Giro=5; seq_b_single=21;
    This is strange for me since my own sequence never has this detailed header in fastq file. So my questions are: 1) Does the fastq file has any information regarding barcodes?

    2) I need to demultiplex this run file. Is there any other way/suggestion available from some experts?

    Note: I have also asked this questions on some other forums but unable to obtain any result since 1 week.

    Best Regards!

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