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Sequencing through the insert



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  • Sequencing through the insert

    Hi I am wondering how common it is for libraries to sequence through the adapter and run out of nucleotide to sequence like in this Illumina video. (https://www.youtube.com/watch?v=bkMiy5lGIqs)

    Is it recommended to analyze the size of you library prior to deciding on read lengths?
    Last edited by kluttgeharm; 07-24-2020, 06:06 AM.

  • #2
    Yes, you definitely want to QC your library for the fragment lengths before sequencing using a Bioanalyzer or equivalent devices.


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