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Nanoarray sequenzing, cluster-plot

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  • Nanoarray sequenzing, cluster-plot

    https://ibb.co/9qbR0st

    I don't understand picture B, it`s an Cluster plot of normalized intensities from a high-density test array with 700nm center-to-center spot distance. This array has 3,4 fold more DNA spots per image than 1,29 micorn arrays. It uses only 4,2 pixels per spot and generates similar raw base discordances.

    Thanks for your help

  • #2
    B looks like an old satay plot from SOLiD sequencing days. They were used to measure signal strength, purity, and phasing. Dots on each axis line are pure colors with no mixed clusters. Dots furthest from the origin on each axis are higher signal or intensity.

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