Hi All,
I am going to prep some RRL to run on the solexa GAIIx and was wondering if anyone had working protocol they could share. I have ordered the enzymes and have read most of the recent publications but the methods are brief.
The main details I would like to clarify are:
Volume of starting DNA
Timing of cleanup steps
Is there a need to run two gel through the protocol, per and post adaptor ligation?
Thank you in advance for your help.
I am going to prep some RRL to run on the solexa GAIIx and was wondering if anyone had working protocol they could share. I have ordered the enzymes and have read most of the recent publications but the methods are brief.
The main details I would like to clarify are:
Volume of starting DNA
Timing of cleanup steps
Is there a need to run two gel through the protocol, per and post adaptor ligation?
Thank you in advance for your help.
Comment