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We didn't do that. In theory, it may cause unbalanced library pool. Some libraries could have very low sequencing reads. -
Pooling prior to PCR amplification is not recommended. It precludes the option of adjusting the concentrations of individual libraries to obtain equal read depth.Leave a comment:
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Hi
I'd like to refresh this thread. Has anyone had success with TruSeq library prep, if you pool your adapter ligated samples prior to the PCR enrichment step?
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Would that also cause jumping errors and waste of sequencing reads?Leave a comment:
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Pooling multiplexed libraries after ligation, before PCR
Does anyone do this with the TruSeq kits? Illumina advertised this feature very strongly in their presentations in my area, as a huge time saving feature of the TruSeq kits as compared to the older sample prep kits... yet the product manual doesn't say anything about it. I'm a bit worried about uneven amplification of the libraries during a pooled PCR. Any comments?
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