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AmpliSeq vs TruSeq custom amplicon

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  • AmpliSeq vs TruSeq custom amplicon

    I have three genes that I want to capture and sequence in an initial 250 patient samples. I wanted to do a comparison between Illumina's new custom amplicon/golden gate and Ion Torrent's AmpliSeq.

    It looks best to wait until they launch in March. Fair enough rules for early access?

    http://www.ampliseq.com/ampliseq/wizard

    5. LTC retains all right, title and interest in and to any Custom Product provided hereunder and grants you a non-exclusive, non-transferable, non-sublicensable license to use such Custom Product solely for your internal research purposes. Commercial use of such Custom Product is expressly prohibited.

    6. Within thirty (30) days of a request by LTC and to the extent so requested, you will provide data resulting from your analysis or testing of the Products (collectively, the “Test Results”), or a summary thereof, in writing to LTC. You shall permit LTC to use the Test Results for Product improvement purposes.

    7. You shall not publish, disseminate, or otherwise share the Test Results relating to the Products without LTC’s prior express written consent.

    8. Any information provided by LTC related to the Products, including, without limitation, any protocols or data, is provided “as is” and shall not be supported by LTC.

    9. You shall promptly disclose any improvement that relates to (a) configuration, composition, use, manufacturing, layout, or packaging of any Product; and/or (b) software and techniques relating to the extraction, manipulation, processing, analysis or storage of data ("Product Improvements"). "Product Improvements" shall not include data generated using the Product or discoveries derived therefrom (except as expressly set forth in (a) – (b) above). Except to the extent prohibited by applicable laws, you hereby grant to LTC a non-exclusive, worldwide, fully sublicensable, fully paid-up, royalty-free, irrevocable, perpetual license to all Product Improvements.

    10. You will be signed up to wash and wax the Ion Bus. http://www.iontorrent.com/ionbus?CID=0003GZ_20120207
    Last edited by epistatic; 02-08-2012, 10:51 AM.

  • #2
    Interesting... reminds me of old Taqman days... When it was time to actual licence things, AB would never get back to us or even transfer our demans properly. That being said, there are other sample prep solutions out there. Have you checked into the Access Array from fluidigm, there is a capital cost but if the sample size is high enough, the routine work becomes inexpensive.

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    • #3
      We are launching our GenomePooling technology, that allows users to pool multiplexed tagged genomes of up to 384 samples, followed by region of interest extraction and NGS on any platform. Pooled genome libraries can be reused for subsequent extractions, thus removing the need for repeat sample preps.

      If you are interested go to our website and contact us:

      http://www.populationgenetics.com/

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      • #4
        There are two ways to create custom amplicon libraries for use in your Sequencing experiments: AmpliSeq and TruSeq. With our guide here, we compare their features and functionality, help you choose the best one for your application, and provide a step-by-step process to get you started. Also you can check https://bitcoingamble.net/dogecoin-casinos/ for the latest new casino games. Let's take a look at these two product types and their strengths, weakness, and possible applications.

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