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You probably need to check amplifiability of your DNA. Using the RNaseP kit will help out with that. Crap in, crap out. -
qubit for quant, but we also use the bioanalyser tp make sure the profile looks as expected (no primer etc.)
JPCLeave a comment:
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We run a Proton and found that we improved our quality by reducing the quantity of DNA that goes into the emPCR by by 20%, we now do this is standard
JPCLeave a comment:
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Cancer panel v2,low quality,316v2
Hi, these days ,I run some 316v2 chips on PGM, and the %low quality show very high, ranged from 27-40%! Can anyone tell me why?
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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