Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Smoki
    replied
    2x 650 is maximum you can get away with.

    With 650 flows, estimate is that mean read length is 346, and 90% of reads should make it to 325.

    Leave a comment:


  • Serratiam
    started a topic Ion Torrent Metagenomics flows

    Ion Torrent Metagenomics flows

    Hello all, I've been looking for optimalization of my metagenomics sequencing process.
    I usually use 316chip on PGM and as default in IonTorrent 16S metagenomics workflow inumber of flows is setted to 850. It results with half of volume of every dNTP left in bottles and I'm afraid of runnning next sequencing without replacement them to new. It is really increasing my sequencing cost because I need to use new inicialization chemistry for each sequencing. I use about 400 bp length fragments but in real its average is about 250 bp(as I can see in the end sequencing summary). Can I decrease number of flows to maybe about 650 to reach two sequencing processes with one inicialization?

Latest Articles

Collapse

  • seqadmin
    Exploring the Dynamics of the Tumor Microenvironment
    by seqadmin




    The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...
    07-08-2024, 03:19 PM
  • seqadmin
    Exploring Human Diversity Through Large-Scale Omics
    by seqadmin


    In 2003, researchers from the Human Genome Project (HGP) announced the most comprehensive genome to date1. Although the genome wasn’t fully completed until nearly 20 years later2, numerous large-scale projects, such as the International HapMap Project and 1000 Genomes Project, continued the HGP's work, capturing extensive variation and genomic diversity within humans. Recently, newer initiatives have significantly increased in scale and expanded beyond genomics, offering a more detailed...
    06-25-2024, 06:43 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 07-10-2024, 07:30 AM
0 responses
23 views
0 likes
Last Post seqadmin  
Started by seqadmin, 07-03-2024, 09:45 AM
0 responses
198 views
0 likes
Last Post seqadmin  
Started by seqadmin, 07-03-2024, 08:54 AM
0 responses
209 views
0 likes
Last Post seqadmin  
Started by seqadmin, 07-02-2024, 03:00 PM
0 responses
191 views
0 likes
Last Post seqadmin  
Working...
X