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  • Afordable 16S libraries by PCR?

    I am trying to establish a protocol to generate 16S libraries using the Ion Amplicon Library Preparation (Fusion Method) that does not rely on the expensive 16S metagenomics + fragment library plus kits from life tech.

    Just wondering whether it would be possible to generate indexed 16S libraries by a single 1 step PCR by just adding the sequence of the Ion Xpress barcode (or others if the case) on each primer pair. This way, I would have 2 fused primer pairs (P-16S FW, P-16S REV, Barcode-16S FW, Barcode-16S REV) for each sample.

    Any help on this would be much appreciated!

    Cheers
    Last edited by jocasbal; 08-20-2021, 03:20 AM. Reason: typo

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