Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • G400 fastq header description?

    Hello,

    I am doing a comparison of duplicated reads on Illumina NovaSeq vs MGI G400 by checking the distribution of reads on the flowcell. I noticed the fastq header from the G400 is quite different from that of the NovaSeq.

    For example, one of the headers is:

    @F350009384L1C001R0010008170/1
    which I interpret as "flowcell id: F350009384", "lane: L1", "tile column: C001", "tile row: R001", "read: /1" and the remaining part "0008170" is unclear to me. Could this be the X and Y coordinates of the cluster/DNB?

    Would love to hear if my interpretation is correct. Thanks!

  • #2
    Complete genomics fastq header format has the following information:
    • @<Flowcell-id><LaneNo><Column><Row><FieldofView></readNumber>
    • Read1:
      • @F350009384 L1 C001 R001 00001290 /1
    • Read2:
      • @F350009384 L1 C001 R001 00001266 /2
    ​That said a recent basecalling software update from CG now has the ability of producing Illumina-like headers.

    Comment


    • #3
      Thank you everyone! MGI representatives told me the cluster/DNB location is not available in their fastq format. I would love to see if anyone used their new basecalling software as mentioned by GenoMax!

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Genetic Variation in Immunogenetics and Antibody Diversity
        by seqadmin



        The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...
        11-06-2024, 07:24 PM
      • seqadmin
        Choosing Between NGS and qPCR
        by seqadmin



        Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
        10-18-2024, 07:11 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 11-08-2024, 11:09 AM
      0 responses
      219 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 11-08-2024, 06:13 AM
      0 responses
      161 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 11-01-2024, 06:09 AM
      0 responses
      80 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 10-30-2024, 05:31 AM
      0 responses
      27 views
      0 likes
      Last Post seqadmin  
      Working...
      X