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  • rlfr
    replied
    You will always loose pores when flushing. Prepping and adding the newly made library wasn't an option?

    Leave a comment:


  • GiacomoBernardi
    started a topic Flushing flow cells

    Flushing flow cells

    I was wondering if anyone has actually ever successfully flushed a flow cell. I have tried three or four times and it has always failed. Today, I started with a flow cell that had 1300 pores. I loaded a library. As it turns out, I had made a huge mistake, I made my library without using ligase in the ligation step (I promise this is the first time it happened in very many libraries). IN any case, after about 5 minutes into the run, I realized it was a mess and then also realized my mistake. I thought, no problem, I'll just flush the cell and reload a new library. I flushed the cell, waited 60 minutes, as is required, and checked the flow cell. It had only 580 pores available, so it was not possible to reload it. Really? Don't get me wrong, I love ONT, but I am just baffled at the flushing story....

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  • seqadmin
    Multiomics Techniques Advancing Disease Research
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    New and advanced multiomics tools and technologies have opened new avenues of research and markedly enhanced various disciplines such as disease research and precision medicine1. The practice of merging diverse data from various ‘omes increasingly provides a more holistic understanding of biological systems. As Maddison Masaeli, Co-Founder and CEO at Deepcell, aptly noted, “You can't explain biology in its complex form with one modality.”

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