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You will always loose pores when flushing. Prepping and adding the newly made library wasn't an option? -
Flushing flow cells
I was wondering if anyone has actually ever successfully flushed a flow cell. I have tried three or four times and it has always failed. Today, I started with a flow cell that had 1300 pores. I loaded a library. As it turns out, I had made a huge mistake, I made my library without using ligase in the ligation step (I promise this is the first time it happened in very many libraries). IN any case, after about 5 minutes into the run, I realized it was a mess and then also realized my mistake. I thought, no problem, I'll just flush the cell and reload a new library. I flushed the cell, waited 60 minutes, as is required, and checked the flow cell. It had only 580 pores available, so it was not possible to reload it. Really? Don't get me wrong, I love ONT, but I am just baffled at the flushing story....
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Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
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The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben MartÃnez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...11-06-2024, 07:24 PM
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