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  • Anyone have experience with the new Sequel chemistry and software?

    PacBio announced an update to the Sequel software and chemistry about a month ago. Have any of the Sequel users here had any experience with it? Does it solve the read length and loading issues? Or is it still more practical to go with a RSII for sequencing.

    Thank you

  • #2
    I just attended the PacBio conference today. It was fun! Lots of interesting presentations (mainly about how PacBio achieves assemblies with L50's 1000x longer than Illumina). And I learned something that I was not aware of before - the Sequel platform has not actually been released yet. It's still in development.

    Therefore - at present, yes, it is more practical to go with RSII for sequencing.

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    • #3
      I thought the new update finally released the platform for production use? Or is this still not the case? Will we have to wait until the end of year update until service providers start services?

      Has anyone tested the performance of the new chemistry yet?

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      • #4
        Originally posted by Brian Bushnell View Post
        - the Sequel platform has not actually been released yet. It's still in development.
        Im the product manager for the Sequel system and gave the morning talk at UGM highlighting our latest results on the system. Apologies if I mislead you on the status of our system. The Sequel system has been released and started shipping end of last year, see our Q1 financial Press Release

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        • #5
          I cannot remember any tweet on your talk and wonder if it's recording is available on line for viewing.

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          • #6
            Its not recorded as we have not been recording talks at our user group meetings.

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            • #7
              Originally posted by mbadgett View Post
              Im the product manager for the Sequel system.
              So the burning question on everyone's mind. When do we get to see real data from Sequel in wild?

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              • #8
                Originally posted by GenoMax View Post
                So the burning question on everyone's mind. When do we get to see real data from Sequel in wild?
                Check out today's blog: http://http://www.pacb.com/blog/sequ...nome-assembly/

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                • #9
                  Is this using the August chemistry update or is it using the planned EOY chemistry update?

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                  • #10
                    It is currently in early access testing and released broadly thereafter, before the year end chemistry.

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                    • #11
                      If I am correct the Sequel data in the blog are not from current chemistry.

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                      • #12
                        Originally posted by nucacidhunter View Post
                        If I am correct the Sequel data in the blog are not from current chemistry.
                        That data will be available next year

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                        • #13
                          Originally posted by mbadgett View Post
                          Thanks. Does this mean the NDA (if there was one in place) is now lifted and others can start releasing their data?

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                          • #14
                            There has never been an NDA for Sequel or its data. Also the updated chemistry is available for order and will start shipping to customers this week.

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                            • #15
                              I wonder if this chemistry release is the last one for this year or there will be another chemistry or SMRT cell update before next year.

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