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Anyone have experience with the new Sequel chemistry and software?

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  • #16
    seems like there is a surprising amount of misinformation. Thanks for clarifying.

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    • #17
      Hi all, has anyone tested the performance of the novel chemistry yet? Any experiences regarding loading efficiency, readoutput, dataquality and so on? I would really, appreciate any kind of information from all you sequel users out there!

      Thanks!

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      • #18
        Hi All,
        we have only run E.coli libraries with the new chemistry so far.
        http://dnatech.genomecenter.ucdavis....pacbio-sequel/
        Last edited by DNATECH; 11-11-2016, 12:15 PM.

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        • #19
          Have a look at https://downloads.pacbcloud.com/publ...opsisDemoData/

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          • #20
            Hi all and thanks for your suggestions! I already recognised pacBios Arabidopsis release. However, the Sequel-data comes in a "new" format (*.bam) and one needs to use smrt-link to visualise the data. Samtools seems not to be feasible to have a look into the binary files. Is anyone aware of a workaround to open or even convert the files? Maybe to make them suitable for import into smrt-portal?
            @DNATECH: Very helpful info!

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            • #21
              Originally posted by Gorgon_VZ View Post
              Hi all and thanks for your suggestions! I already recognised pacBios Arabidopsis release. However, the Sequel-data comes in a "new" format (*.bam) and one needs to use smrt-link to visualise the data. Samtools seems not to be feasible to have a look into the binary files. Is anyone aware of a workaround to open or even convert the files? Maybe to make them suitable for import into smrt-portal?
              @DNATECH: Very helpful info!
              What issue are you having with samtools?

              the new SEQUEL *.bam files are 100% compatible with samtools, we routinely use it internally and it's included as part of SMRTLink

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              • #22
                Does anyone have any experience with the new Sequel chemistry on their own system?

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