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PacBio Sequel demultiplexing



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  • PacBio Sequel demultiplexing


    Anyone who has experience with demultiplexing Sequel data via SMRT link command line options?

    What I've done so far is to follow the "Barcode splitting for command-line analysis (SMRT Analysis v3.1)" section in


    and then use the demultiplex.py at https://github.com/PacificBiosciences/SMRT-Link

    But when I do assembly with the demultiplexed data the results I get are just strange and not comparable to the assembly results I get from the same machine without demultiplexing. So I'm guessing that I'm doing something wrong.


  • #2
    Reach out to PacBio tech support directly. You may get a more rapid response that way. Sequel is so new that not many on this site have worked with that data as yet.


    • #3
      I tried that already, have been waiting for three days now. I guess they're very busy for the upcoming software and chemistry release.


      • #4
        Ah well. People from PacBio do participate here so there is a chance you will see something before end of today (California, pacific time).


        • #5
          Hi Ali,

          We have a record of your case with PacBio tech support. It is case #52474 and was closed on 10/21.


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