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  • Pooling PacBio libraries

    Hello all, glad to join the seqanswers community! I am a new PacBio user and hoping to use this forum to get some advice on pooling and sequencing libraries.

    The project I am currently planning involves the sequencing of 5 HLA loci. The PCR for HLA-DRB1 produces two products of about 2.5kb and 5kb (which must be pooled together), while the rest are single products of between 3.4kb and 5.8kb.

    I am concerned about the pooling of a smaller 2.5kb product with 5+kb ones. If anyone has experience of this protocol or similar, and achieved sufficient coverage, it would be great to chat! I couldn't find a current thread on this subject so apologies if I've missed one.

    Thanks in advance,

    Hannah
    Last edited by GenoMax; 06-12-2020, 05:35 AM. Reason: Make the thread noticeable

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