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Hi all,
I want to ask if anyone ever experienced randomly small G and C peaks in their sequence. We are using BDT1.1 on a AB3130 and AB310 sequencer. Since a while we observe every here and than very tiny G and C peaks in an otherwise normal and good sequence. This is reproducible on both sequencers. Sometimes the basecaller even regards it as a gap/deletion.
- Changing the LOT of the BDT1.1 did not help.
- Different PCR-Kits and conditions did not help
- Same observation on two sequencers.
- Changing the sequncing cycle programm did not help.
https://drive.google.com/file/d/0B1U...ew?usp=sharing
I am thankful for any idea.
-mike-
I want to ask if anyone ever experienced randomly small G and C peaks in their sequence. We are using BDT1.1 on a AB3130 and AB310 sequencer. Since a while we observe every here and than very tiny G and C peaks in an otherwise normal and good sequence. This is reproducible on both sequencers. Sometimes the basecaller even regards it as a gap/deletion.
- Changing the LOT of the BDT1.1 did not help.
- Different PCR-Kits and conditions did not help
- Same observation on two sequencers.
- Changing the sequncing cycle programm did not help.
https://drive.google.com/file/d/0B1U...ew?usp=sharing
I am thankful for any idea.
-mike-