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  • Devidas Thombare
    replied
    Hi sir/madam,
    I'm analyzing the results of few PCR product sequenced by Sanger method. I have the result using forward and reverse primer. Because the PCR products aren't too long, there are regions that overlaps, so I want to merge this to overlapping sequences to get only one big sequence.

    Are there any online tool to do this?

    Leave a comment:


  • GenoMax
    replied
    There is nothing special about plasmids. https://en.wikipedia.org/wiki/Sanger_sequencing or https://www.youtube.com/watch?v=vK-HlMaitnE and similar videos.

    Leave a comment:


  • Sunita
    started a topic Sanger sequencing

    Sanger sequencing

    Can anyone help me in understanding how plasmids are sequenced using the chain termination method?
    Thanks.

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