Header Leaderboard Ad


SEQanswers Roundup 12/14: Polonator info, Nimblegen, Nucleotides, Terminology ?'s



No announcement yet.
  • Filter
  • Time
  • Show
Clear All
new posts

  • SEQanswers Roundup 12/14: Polonator info, Nimblegen, Nucleotides, Terminology ?'s

    Update for the week ending 12/14:

    SEQanswers.com Forum has had its busiest week yet, when for the first time, community members have made more new posts than I have! I'm going to try to put together a weekly (maybe bi-weekly?) post which highlights the topics being discussed on our forums.

    1. In response to my call for information about the George Church next-gen sequencer, member #11 pmcget posted some very interesting insider information about the "Polonator". (See post #4 in this thread)

    2. New user colicoli shares some detailed workflow information about Nimblegen array enrichment and competing technologies from the Church consortium (See post #4 in this thread)

    3. User questions! We're starting to get user questions which I cannot thoroughly answer on my own, so this is where community participation will be key. The new General forum has two questions: Moscow user Michael L. Altshuler asks about the use of specific terminology for describing sequencing metrics such as accuracy and coverage, and colicoli poses some important questions regarding the costs of the specific nucleotides used in the next gen platforms.

    4. New forums. In response to user suggestions, I have opened a Bioinformatics forum for discussions of computational issues/questions/problems related to next-gen sequencing. To initiate the forum, I posted a summary of a new paper which describes a next-gen sequencing assembly algorithm which was specifically coded to run on economical graphics processors. Cool stuff. I've also created a General forum to hold questions/topics that don't fit in any of the other subdivisions.

    OK that's it for this week. I want to thank everyone who has contributed to the site, and to everyone who is reading the site. I would encourage you to share the site with colleagues that might be interested.

Latest Articles


  • seqadmin
    A Brief Overview and Common Challenges in Single-cell Sequencing Analysis
    by seqadmin

    ​​​​​​The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i...

    01-24-2023, 01:19 PM
  • seqadmin
    Introduction to Single-Cell Sequencing
    by seqadmin
    Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.

    The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes
    01-09-2023, 03:10 PM