-
Can someone please kindly give expertise on how I should go about pooling my samples. I have done library preparation for 80 samples INDIVIDUALLY (yes...06-24-2022, 12:50 PM -
Reminder! We are less than one month out from the NCTR Indel Calling from Oncopanel Sequencing Data Challenge submission deadline! There’s still time...06-22-2022, 12:41 PM
-
My most recent MiSeq (Nano v2) run failed with the following error: No usable signal found, it is possible clustering has failed.
I had a look at...You do not have permission to view this gallery.
This gallery has 2 photos.06-22-2022, 06:52 AM
No content found
Please log in to your account to view your subscribed posts.
| Directory | Topics | Posts | Last Post |
|---|---|---|---|
|
Introductions
New here? Stop in and introduce yourself. Where you are, what you work on, etc.
Topics: 4,827
Posts: 7,025
Last Post:
Bacterial WGS on Ion Torrent
|
4,827 | 7,025 |
|
|
General
Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
Topics: 2,560
Posts: 7,756
Last Post:
understanding VCF ids
|
2,560 | 7,756 |
|
|
Core Facilities
Dealing with customer samples, data, and the challenges that come with both?
Topics: 128
Posts: 579
Last Post:
syndip dataset for benchmark variant
|
128 | 579 |
|
|
Events / Conferences
Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)
Topics: 710
Posts: 1,208
|
710 | 1,208 |
by alyse_c
06-22-2022, 12:41 PM
|
|
Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
Topics: 41,772
Posts: 42,456
|
41,772 | 42,456 |
by pvaloblogs
06-17-2022, 09:50 PM
|
| Sequencing Technologies/Companies | |||
|
454 Pyrosequencing
Pyrosequencing in picotiter plates, custom arrays for enrichment/decomplexing. (Roche)
Topics: 762
Posts: 3,982
Last Post:
Roche 454 RL adapters.. help!!!
|
762 | 3,982 |
|
|
MGISEQ (FKA Complete Genomics)
Service-based whole human genome sequencing with probe ligation chemistry.
Topics: 50
Posts: 160
|
50 | 160 |
|
|
Helicos / Direct Genomics
True Single Molecule Sequencing, originally by Helicos, tech acquired by Direct Genomics
Topics: 29
Posts: 152
Last Post:
Download of example Data Helicos
|
29 | 152 |
|
|
Illumina/Solexa
Bridged amplification & clustering followed by sequencing by synthesis. (Genome Analyzer / HiSeq / MiSeq)
Topics: 2,981
Posts: 16,515
|
2,981 | 16,515 |
|
|
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Life Technologies)
Topics: 388
Posts: 2,212
Last Post:
New Ion Torrent Sequencer?
|
388 | 2,212 |
|
|
Oxford Nanopore
Single molecule sequencing via a protein nanopore, presently on the MinION.
Topics: 105
Posts: 563
Last Post:
Priming Flongle Flowcell
|
105 | 563 |
|
|
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures
Topics: 382
Posts: 2,171
Last Post:
In-person event: Discoveries Roadshow
|
382 | 2,171 |
|
|
Polonator
"Open Source" instrument and sequencing by ligation chemistry (Danaher Motion-Dover)
Topics: 5
Posts: 68
|
5 | 68 |
|
|
Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 75
Posts: 266
Last Post:
ABI 3130xl 50 cm Array NOS Lifetime
|
75 | 266 |
|
|
SOLiD
Sequencing by Oligo Ligation/Detection (Life Technologies)
Topics: 462
Posts: 2,443
Last Post:
regarding the values of quality
|
462 | 2,443 |
|
|
The Pipeline
Sequencing technologies without a commercially released platform (Oxford Nanopore, Halcyon Molecular, etc.)
Topics: 79
Posts: 577
Last Post:
List of next-next gen...
|
79 | 577 |
|
|
Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
Topics: 72
Posts: 474
Last Post:
Buy pure stevia powder from online
|
72 | 474 |
|
|
Vendor Forum
Registered SEQanswers sponsors/vendors can post commercial content here. Please support our sponsors!
Topics: 329
Posts: 806
|
329 | 806 |
|
| Bioinformatics | |||
|
Bioinformatics
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
Topics: 21,835
Posts: 102,591
Last Post:
Good annotation editor?
|
21,835 | 102,591 |
|
|
Open Genomics Engine Project
Open discussion forum for Open Genomics Engine contributors and users
Topics: 9
Posts: 40
|
9 | 40 |
|
| Jobs Forums | |||
|
Industry Jobs!
Job listings at companies that sponsor SEQanswers.com! Interested in posting a job? Contact [email protected] for access.
Topics: 215
Posts: 246
Last Post:
Senior Scientist, NGS
|
215 | 246 |
|
|
Academic/Non-Profit Jobs
Post and look for academic, non-profit, or other non-industrial jobs here.
Topics: 1,541
Posts: 1,764
|
1,541 | 1,764 |
|
| Applications Forums | |||
|
Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,149
Posts: 9,743
|
2,149 | 9,743 |
by aliceseq
06-24-2022, 12:50 PM
|
|
Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
Topics: 277
Posts: 987
|
277 | 987 |
by Zw1224
05-26-2022, 03:05 AM
|
|
De novo discovery
Wandering without a reference? Post here
Topics: 340
Posts: 1,712
|
340 | 1,712 |
by Rezaeir
08-16-2020, 08:43 AM
|
|
Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 261
Posts: 827
Last Post:
Shallow shotgun metagenomics
|
261 | 827 |
|
|
Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 351
Posts: 1,569
|
351 | 1,569 |
|
|
RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,836
Posts: 11,352
|
2,836 | 11,352 |
by scienceguy
06-09-2022, 11:28 AM
|
|
Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 37
Posts: 102
|
37 | 102 |
|
| Regional/Local Communities | |||
|
Topics: 43
Posts: 70
|
43 | 70 |
by narain
04-25-2022, 07:28 AM
|
| Personalized Genomics | |||
|
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
Topics: 58
Posts: 294
Last Post:
Bione Genetic Testing for Cancer
|
58 | 294 |
|
| Site News | |||
|
Site Announcements
Announcements regarding SEQanswers
Topics: 46
Posts: 198
|
46 | 198 |
|
|
Site Feedback/Suggestions
Please post all feedback or suggestions about the site in this forum.
Topics: 48
Posts: 661
Last Post:
Cannot view attachments?
|
48 | 661 |
|
|
Wiki Discussion
Discussions about SEQwiki. Problems, suggestions, offers to help...
Topics: 36
Posts: 279
Last Post:
Information about Wikipedia?
|
36 | 279 |
|
|
Webinar Series
Planning and feedback subforum for planning sequencing webinar episodes
Topics: 25
Posts: 41
|
25 | 41 |
|
There are currently 144 users online. 1 members and 143 guests.
Most users ever online was 14,951 at 02:11 PM on 05-02-2022.
-
Can someone please kindly give expertise on how I should go about pooling my samples. I have done library preparation for 80 samples INDIVIDUALLY (yes...06-24-2022, 12:50 PM
-
RNA-Seq Data Analysis Workshop
Quality Control, Read Mapping, Visualization and Downstream Analyses
When? October...05-31-2022, 02:24 AM -
Reminder! We are less than one month out from the NCTR Indel Calling from Oncopanel Sequencing Data Challenge submission deadline! There’s still time...06-22-2022, 12:41 PM
-
My most recent MiSeq (Nano v2) run failed with the following error: No usable signal found, it is possible clustering has failed.
I had a look at...You do not have permission to view this gallery.
This gallery has 2 photos.06-22-2022, 06:52 AM -
Hi,
Can anyone please help me understand why i get ID the same names in VCF file (mine is SV from svaba) with name:1 name:2, .Isn't that the same...06-18-2022, 11:57 PM