Use your standard DNA variant caller for SNP calling in Bisulfite-Seq data
Until now, specialised tools are required in order to access DNA...
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Channel: Bioinformatics
Today, 01:03 AM -
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by seaureDie Leute haben eine weiche Ecke für diese Kleider entwickelt und sie tragen sie bei jeder Gelegenheit. Einige der beliebten Blumenkleider, die auf dem...
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Channel: Introductions
Yesterday, 08:57 PM -
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by aliceseqCan someone please kindly give expertise on how I should go about pooling my samples. I have done library preparation for 80 samples INDIVIDUALLY (yes...
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Channel: Sample Prep / Library Generation
06-24-2022, 12:50 PM -
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Introductions
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Hi all
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General
Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
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understanding VCF ids
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Dealing with customer samples, data, and the challenges that come with both?
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syndip dataset for benchmark variant
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Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)
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by alyse_c
06-22-2022, 12:41 PM
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Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
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by pvaloblogs
06-26-2022, 08:43 AM
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Sequencing Technologies/Companies | |||
454 Pyrosequencing
Pyrosequencing in picotiter plates, custom arrays for enrichment/decomplexing. (Roche)
Topics: 762
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Last Post:
Roche 454 RL adapters.. help!!!
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762 | 3,982 |
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MGISEQ (FKA Complete Genomics)
Service-based whole human genome sequencing with probe ligation chemistry.
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Posts: 160
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50 | 160 |
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Helicos / Direct Genomics
True Single Molecule Sequencing, originally by Helicos, tech acquired by Direct Genomics
Topics: 29
Posts: 152
Last Post:
Download of example Data Helicos
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29 | 152 | |
Illumina/Solexa
Bridged amplification & clustering followed by sequencing by synthesis. (Genome Analyzer / HiSeq / MiSeq)
Topics: 2,981
Posts: 16,515
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2,981 | 16,515 | |
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Life Technologies)
Topics: 388
Posts: 2,212
Last Post:
New Ion Torrent Sequencer?
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388 | 2,212 | |
Oxford Nanopore
Single molecule sequencing via a protein nanopore, presently on the MinION.
Topics: 105
Posts: 563
Last Post:
Priming Flongle Flowcell
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105 | 563 | |
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures
Topics: 382
Posts: 2,171
Last Post:
In-person event: Discoveries Roadshow
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Polonator
"Open Source" instrument and sequencing by ligation chemistry (Danaher Motion-Dover)
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Posts: 68
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5 | 68 | |
Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
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ABI 3130xl 50 cm Array NOS Lifetime
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SOLiD
Sequencing by Oligo Ligation/Detection (Life Technologies)
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regarding the values of quality
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462 | 2,443 | |
The Pipeline
Sequencing technologies without a commercially released platform (Oxford Nanopore, Halcyon Molecular, etc.)
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Last Post:
List of next-next gen...
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Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
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Buy pure stevia powder from online
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Bioinformatics | |||
Bioinformatics
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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Last Post:
Good annotation editor?
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Open Genomics Engine Project
Open discussion forum for Open Genomics Engine contributors and users
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Jobs Forums | |||
Industry Jobs!
Job listings at companies that sponsor SEQanswers.com! Interested in posting a job? Contact [email protected] for access.
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Senior Scientist, NGS
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Applications Forums | |||
Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Posts: 9,743
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2,149 | 9,743 |
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by aliceseq
06-24-2022, 12:50 PM
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
Topics: 277
Posts: 987
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277 | 987 |
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by Zw1224
05-26-2022, 03:05 AM
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De novo discovery
Wandering without a reference? Post here
Topics: 340
Posts: 1,712
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340 | 1,712 |
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by Rezaeir
08-16-2020, 08:43 AM
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Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 261
Posts: 827
Last Post:
Shallow shotgun metagenomics
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261 | 827 | |
Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 351
Posts: 1,569
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351 | 1,569 | |
RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,836
Posts: 11,352
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2,836 | 11,352 |
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by scienceguy
06-09-2022, 11:28 AM
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Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 37
Posts: 102
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Regional/Local Communities | |||
Topics: 43
Posts: 70
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43 | 70 |
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by narain
04-25-2022, 07:28 AM
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Personalized Genomics | |||
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
Topics: 58
Posts: 294
Last Post:
Bione Genetic Testing for Cancer
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Site Announcements
Announcements regarding SEQanswers
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Site Feedback/Suggestions
Please post all feedback or suggestions about the site in this forum.
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Cannot view attachments?
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Wiki Discussion
Discussions about SEQwiki. Problems, suggestions, offers to help...
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Information about Wikipedia?
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Webinar Series
Planning and feedback subforum for planning sequencing webinar episodes
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Use your standard DNA variant caller for SNP calling in Bisulfite-Seq data
Until now, specialised tools are required in order to access DNA...-
Channel: Bioinformatics
Today, 01:03 AM -
-
by seaureDie Leute haben eine weiche Ecke für diese Kleider entwickelt und sie tragen sie bei jeder Gelegenheit. Einige der beliebten Blumenkleider, die auf dem...
-
Channel: Introductions
Yesterday, 08:57 PM -
-
by aliceseqCan someone please kindly give expertise on how I should go about pooling my samples. I have done library preparation for 80 samples INDIVIDUALLY (yes...
-
Channel: Sample Prep / Library Generation
06-24-2022, 12:50 PM -
-
by amstrong32Marpac Dohm Classic White Noise Machine review
Have you ever had trouble sleeping because it was too quiet? Or have you ever tried to fall...-
Channel: Introductions
09-24-2020, 04:49 AM -
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by MaunoProtein Structure and Bioinformatics Group (http://structure.bmc.lu.se/) at Lund University, Sweden is looking for a postdoctoral fellow to work in Vetenskapsrådet...
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Channel: Academic/Non-Profit Jobs
11-18-2019, 04:36 AM -