Hey naamasht,
Honestly, I don't know if it will make a difference because normally having problems with those primers causes the opposite...
Ben3
Member
Last Activity: 05-18-2023, 12:03 PM
Joined: 09-16-2022
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naamasht it sounds like you've already checked everything. But when you said you tried using new sequencing primers, do you mean new primers (16S V4 amplification...Leave a comment:
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Tarie I couldn't find it on the website, and that's where I'm pretty sure I got it before. I recommend reaching out to the email listed on this contact...Leave a comment:
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Hello Tarie, are you using a specific excel spreadsheet for this? The last time I did it, I remember using one from the KAPA website that did all the...Leave a comment:
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valeriagomez99 yeah that doesn't sound good. Have you spoken to Illumina about this? I'm going to assume it's something wrong with the MiSeq because clearly,...Leave a comment:
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valeriagomez99 has this happened more than once? I can't understand why the quality would drop so low. Also, what kind of samples were you running?...Leave a comment:
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Malfet well, I've honestly never had good luck when I was combining libraries unless I did a nano run on a MiSeq to balance them out. Hopefully it looks...Leave a comment:
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Malfet that's very strange. My immediate suspicion would be the Cut&Tag libraries that were included. Have you run them on another instrument before?...Leave a comment:
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