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Introductions
New here? Stop in and introduce yourself. Where you are, what you work on, etc.
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2,277 4,401
Sequencing Resources
SEQanswers sponsors/vendors can post commercial content here with approval from admin. Please support our sponsors!
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357 825
seqadmin
News
News about the latest advances and discoveries in sequencing and related technologies.
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284 286
Core Facilities
Dealing with customer samples, data, and the challenges that come with both?
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127 577
General
Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
Topics: 2,615 Posts: 7,818
2,615 7,818
Webinar Series
Upcoming and on-demand webinars by SEQanswers and its partners.
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Last Post: On-Demand Webinars
40 56
seqadmin
Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
Topics: 41,653 Posts: 42,332
41,653 42,332
Sequencing Technologies/Companies
Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 53 Posts: 165
53 165
Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
Topics: 2 Posts: 5
2 5
Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
Topics: 3,057 Posts: 16,723
3,057 16,723
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
Topics: 392 Posts: 2,174
392 2,174
Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
Topics: 118 Posts: 599
118 599
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
Topics: 385 Posts: 2,176
385 2,176
Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
Topics: 0 Posts: 0
0 0
Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
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0 0
Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 76 Posts: 278
76 278
Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
Topics: 79 Posts: 480
79 480
Archived Forums
A place for discussion of older forums and sequencing technologies.
Topics: 1,361 Posts: 7,278
Last Post: Rapid Libraries
1,361 7,278
pmiguel
by pmiguel
Sub-Forums:
UK - Cambridge (37/67)
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454 Pyrosequencing (743/3,966)
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Bioinformatics
Bioinformatics
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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22,113 103,083
Events / Conferences
Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)
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782 1,297
Jobs Forums
Industry Jobs!
Job listings at companies that sponsor SEQanswers.com! Interested in posting a job? Contact admin@ for access.
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222 253
vivianshaw
Academic/Non-Profit Jobs
Post and look for academic, non-profit, or other non-industrial jobs here.
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1,606 1,822
Applications Forums
Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,202 Posts: 9,902
2,202 9,902
Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
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Last Post: Understanding UMAP
7 14
fchatonnet
Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
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278 991
De novo discovery
Wandering without a reference? Post here
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342 1,701
Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 273 Posts: 852
273 852
Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 357 Posts: 1,578
357 1,578
RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,873 Posts: 11,432
2,873 11,432
Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 42 Posts: 107
42 107
Personalized Genomics
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
Topics: 58 Posts: 291
58 291
Site News
Site Announcements
Announcements regarding SEQanswers
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seqadmin
Site Feedback/Suggestions
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48 657

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  • seqadmin
    On-Demand Webinars
    by seqadmin
    This fall was packed with four must-see webinars! If you missed out, they’re all available on demand for you to watch at your convenience.

    ...
    11-04-2024, 08:07 AM

Recent Articles

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  • seqadmin
    Genetic Variation in Immunogenetics and Antibody Diversity



    The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...
    11-06-2024, 07:24 PM
  • seqadmin
    Choosing Between NGS and qPCR



    Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
    10-18-2024, 07:11 AM
  • seqadmin
    Non-Coding RNA Research and Technologies




    Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.

    Nobel Prize for MicroRNA Discovery
    This week,...
    10-07-2024, 08:07 AM

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