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Hi everyone, I’m conducting a short 5-minute survey to better understand what matters most to researchers like you when selecting RNA-seq kits and services....Today, 05:15 AM -
Sample preparation is a critical step in next-generation sequencing (NGS) that requires precision and innovation to address common challenges. Our new...11-27-2024, 02:41 PM
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I'm analyzing fungal ITS amplicon data from gut samples. However, the data quality isn't very good—there's a noticeable drop in...11-25-2024, 07:23 PM
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Sequencing Resources
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Advances in NGS Sample Prep
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News about the latest advances and discoveries in sequencing and related technologies.
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by seqadmin
11-22-2024, 07:36 AM
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Core Facilities
Dealing with customer samples, data, and the challenges that come with both?
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post-bisulphite conversion QC check
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11-13-2024, 08:36 PM
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03-27-2023, 11:17 PM
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Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
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G400 fastq header description?
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Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
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Weird 16S custom primer issue on Aviti runs
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Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
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Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
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Ion Ampliseq library prep query
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Single-molecule sequencing via a protein nanopore.
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Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
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by Osingh
02-20-2024, 05:21 AM
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Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
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Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
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Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
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A place for discussion of older forums and sequencing technologies.
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Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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Split VCF file based on a list of samples
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11-21-2024, 08:04 AM
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by AI4BI_CDT
10-28-2024, 06:36 AM
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Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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DNA turns black over time - contamination?
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Techniques, discussions, and applications of single cell sequencing.
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Understanding UMAP
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
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De novo discovery
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Hifiasm and sex chromosomes from XY specimen
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Metagenomics
Ever wonder what's growing in that hot spring or glacier?
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Impact of biomass on shotgun sequencing
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Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
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12-09-2023, 09:17 PM
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RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
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How do you select RNA-seq kits?
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Clinical Sequencing
Discuss issues unique to clinical sequencing.
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Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
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Hard to know what is accurate (WES and WGS)
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Hi everyone, I’m conducting a short 5-minute survey to better understand what matters most to researchers like you when selecting RNA-seq kits and services....Today, 05:15 AM
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I have a VCF file and a file with a list of samples. I would like to create a new VCF file only containing the samples on the list.
I could...07-04-2016, 04:45 AM
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This fall was packed with four must-see webinars! If you missed out, they’re all available on demand for you to watch at your convenience.
...11-04-2024, 08:07 AM
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The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...11-06-2024, 07:24 PM -
Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...10-18-2024, 07:11 AM -
Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.
Nobel Prize for MicroRNA Discovery
This week,...10-07-2024, 08:07 AM