Exciting News: ecSeq Bioinformatics 2024 Workshops Are Here!
We're thrilled to announce our diverse range of bioinformatics workshops for...
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Yesterday, 01:31 AM -
Hi everyone,
I am planning a ddRADseq experiment with non-model plants (this means, no reference genome available), and I am wondering how...12-14-2023, 10:13 AM -
Hi everyone,
I am planning a ddRADseq experiment with non-model plants (this means, no reference genome available), and I am wondering how...12-14-2023, 09:58 AM
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News
News about the latest advances and discoveries in sequencing and related technologies.
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by seqadmin
12-14-2023, 08:22 AM
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Core Facilities
Dealing with customer samples, data, and the challenges that come with both?
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syndip dataset for benchmark variant
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Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
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Retrieve molecular barcodes
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by seqadmin
12-13-2023, 10:37 AM
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Literature Watch
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by Biotechvana
03-27-2023, 11:17 PM
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Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 51
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Last Post:
Setting up and using MGI platforms
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Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
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Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
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Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
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Bioanalyzer indispensable?
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Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
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Flushing flow cells
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Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
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July webinar: Cancer Transcriptomics
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Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
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Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
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SeqStudio users?
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Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
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Archived Forums
A place for discussion of older forums and sequencing technologies.
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Rapid Libraries
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Bioinformatics
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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by Jingfang
12-13-2023, 02:46 AM
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Events / Conferences
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NGS Data Analysis Workshops 2024
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Senior Scientist, NGS
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Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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End Repair + A-Tailing - how do they do it?
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Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
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bam file missing barcode and UMI info
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
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De novo discovery
Wandering without a reference? Post here
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infering isoforms in merged transcriptome
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Metagenomics
Ever wonder what's growing in that hot spring or glacier?
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11-30-2023, 05:03 AM
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Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
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12-09-2023, 09:17 PM
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RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
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accesing gene name RNAseq
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Clinical Sequencing
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Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
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Bione Genetic Testing for Cancer
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Exciting News: ecSeq Bioinformatics 2024 Workshops Are Here!
We're thrilled to announce our diverse range of bioinformatics workshops for...Yesterday, 01:31 AM -
Hi everyone,
I am planning a ddRADseq experiment with non-model plants (this means, no reference genome available), and I am wondering how...12-14-2023, 10:13 AM -
Hi everyone,
I am planning a ddRADseq experiment with non-model plants (this means, no reference genome available), and I am wondering how...12-14-2023, 09:58 AM -
Hi all,
from Integrative Genomics Viewer User Guide (Paired-end alignments - IGV Desktop Application), when an inversion shows up in paired-end...12-01-2023, 07:00 AM -
Hi folks,
I'm new here, and I'm in the process of preparing a PCR-generated amplicon sequencing library (composed of many samples) that will...12-12-2023, 09:19 PM
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Join us for an insightful SEQanswers webinar on "Genomic Surveillance and Epidemiology with Next-Generation Sequencing." This session will feature...11-22-2023, 01:38 PM
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In this webinar, DeNovix Application Scientist Ben Capozzoli discusses methods and tips for preparing nuclei samples before single...10-23-2023, 09:22 AM
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Rare diseases affect an estimated 3.5–5.9% of the global population1. Patients dealing with these conditions not only face a direct impact on their health but also frequently encounter challenges in identifying the genetic origins of their disorder. This often leaves them with limited treatment options. However, the field of rare disease diagnosis has seen significant advances with the introduction of next-generation sequencing (NGS). Essential techniques like whole-exome sequencing...12-11-2023, 02:26 PM -
The recent pandemic caused worldwide health, economic, and social disruptions with its reverberations still felt today. A key takeaway from this event is the need for accurate and accessible tools for detecting and tracking infectious diseases. Timely identification is essential for early intervention, managing outbreaks, and preventing their spread. This article reviews several valuable tools employed in the detection and surveillance of infectious diseases....11-27-2023, 01:15 PM -
Microbiome research has led to the discovery of important connections to human and environmental health. Sequencing has become a core investigational tool in microbiome research, a subject that we covered during a recent webinar. Our expert speakers shared a number of advancements including improved experimental workflows, research involving transmission dynamics, and invaluable analysis resources. This article recaps their informative presentations, offering insights...11-09-2023, 07:02 AM