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News
News about the latest advances and discoveries in sequencing and related technologies.
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Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
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2,581 7,782
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Dealing with customer samples, data, and the challenges that come with both?
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127 578
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Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)
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Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
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Sequencing Technologies/Companies
Complete Genomics (MGI)
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
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50 158
Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
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Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
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3,009 16,595
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
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391 2,173
Ben3
by Ben3
Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
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107 573
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
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384 2,175
Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
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Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
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76 274
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Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
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A place for discussion of older forums and sequencing technologies.
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Metagenomics
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harbortc
Epigenetics
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Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
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Clinical Sequencing
Discuss issues unique to clinical sequencing.
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Personalized Genomics
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
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by Sbiljh1
Webinar Series
Planning and feedback subforum for planning sequencing webinar episodes
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Recent Articles

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  • seqadmin
    Improved Targeted Sequencing: A Comprehensive Guide to Amplicon Sequencing



    Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...
    Yesterday, 01:49 PM
  • seqadmin
    Targeted Sequencing: Choosing Between Hybridization Capture and Amplicon Sequencing




    Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...
    03-10-2023, 05:31 AM
  • seqadmin
    Expert Advice on Automating Your Library Preparations



    Using automation to prepare sequencing libraries isn’t a new concept, and most researchers are aware that there are numerous benefits to automating this process. However, many labs are still hesitant to switch to automation and often believe that it’s not suitable for their lab. To combat these concerns, we’ll cover some of the key advantages, review the most important considerations, and get real-world advice from automation experts to remove any lingering anxieties....
    02-21-2023, 02:14 PM

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