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by AmarkHi everyone, I’m conducting a short 5-minute survey to better understand what matters most to researchers like you when selecting RNA-seq kits and services....
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Channel: RNA Sequencing
Today, 05:15 AM -
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by seqadminSample preparation is a critical step in next-generation sequencing (NGS) that requires precision and innovation to address common challenges. Our new...
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Channel: Sequencing Resources
11-27-2024, 02:41 PM -
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by ksv6178Hi, all.
I'm analyzing fungal ITS amplicon data from gut samples. However, the data quality isn't very good—there's a noticeable drop in...-
Channel: Bioinformatics
11-25-2024, 07:23 PM -
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11-22-2024, 07:36 AM
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Dealing with customer samples, data, and the challenges that come with both?
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post-bisulphite conversion QC check
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by Biotechvana
03-27-2023, 11:17 PM
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Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
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G400 fastq header description?
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Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
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Bridged amplification & clustering followed by sequencing by synthesis.
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Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
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Single-molecule sequencing via a protein nanopore.
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Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
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02-20-2024, 05:21 AM
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Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
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Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
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Automated dye terminator ("Sanger") chemistries
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10-28-2024, 06:36 AM
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Sample Prep / Library Generation
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Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
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Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
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How do you select RNA-seq kits?
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Personalized Genomics
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by AmarkHi everyone, I’m conducting a short 5-minute survey to better understand what matters most to researchers like you when selecting RNA-seq kits and services....
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Channel: RNA Sequencing
Today, 05:15 AM -
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by LeonDKI have a VCF file and a file with a list of samples. I would like to create a new VCF file only containing the samples on the list.
I could...-
Channel: Bioinformatics
07-04-2016, 04:45 AM -
Webinars
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by seqadminThis fall was packed with four must-see webinars! If you missed out, they’re all available on demand for you to watch at your convenience.
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Channel: Webinar Series
11-04-2024, 08:07 AM -
Recent Articles
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The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...-
Channel: Articles
11-06-2024, 07:24 PM -
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Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...-
Channel: Articles
10-18-2024, 07:11 AM -
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Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.
Nobel Prize for MicroRNA Discovery
This week,...-
Channel: Articles
10-07-2024, 08:07 AM -
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