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For some reason some of my libraries (but not all) that I prepared for ATAC-seq display small fragment peaks (50-70bp) on a Bioanalyzer trace. Im wondering...09-27-2022, 04:38 PM
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syndip dataset for benchmark variant
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Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
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by Dolladen
09-27-2022, 09:16 AM
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| Sequencing Technologies/Companies | |||
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454 Pyrosequencing
Pyrosequencing in picotiter plates, custom arrays for enrichment/decomplexing. (Roche)
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Rapid Libraries
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762 | 3,986 |
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MGISEQ (FKA Complete Genomics)
Service-based whole human genome sequencing with probe ligation chemistry.
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Helicos / Direct Genomics
True Single Molecule Sequencing, originally by Helicos, tech acquired by Direct Genomics
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Illumina/Solexa
Bridged amplification & clustering followed by sequencing by synthesis. (Genome Analyzer / HiSeq / MiSeq)
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Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Life Technologies)
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Oxford Nanopore
Single molecule sequencing via a protein nanopore, presently on the MinION.
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Expired reagents (NEB & ligation)
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Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures
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Polonator
"Open Source" instrument and sequencing by ligation chemistry (Danaher Motion-Dover)
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
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ABI 3130xl 50 cm Array NOS Lifetime
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SOLiD
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Bioinformatics
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Open discussion forum for Open Genomics Engine contributors and users
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by jennywl
09-25-2022, 12:41 PM
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Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
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De novo discovery
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Metagenomics
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Shallow shotgun metagenomics
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Epigenetics
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RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
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by narain
04-25-2022, 07:28 AM
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| Personalized Genomics | |||
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Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
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Do you want coaching at SIEC Education. It is one of the best institutions in the world have been trained by the institute’s test preparation and vocational...Today, 02:04 AM
-
Hi everyone
I have a quick question. Illumina makes its adapter sequences known to the public through documents. Suppose one were to start his/her...10-01-2022, 07:13 PM -
RNA-sequencing (RNA-seq) is a powerful technique for studying the qualitative and quantitative aspects of gene expression, as well as facilitating the...09-27-2022, 08:20 AM
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I'm looking for some help analysing the profile of a 10x genomics 3' scRNA library prep, run on a 1-6000bp HS fragment analyser (attached pic). The second...11-30-2021, 08:26 AM
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Online Course - A Practical Introduction to NGS Data Analysis
Quality Control, Read Mapping, Visualization and DNA Variant Analysis...03-19-2021, 03:36 AM