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Directory Topics Posts Last Post
New here? Stop in and introduce yourself. Where you are, what you work on, etc.
Topics: 2,266 Posts: 4,384
Last Post: Variant filtering
2,266 4,384
Sequencing Resources
SEQanswers sponsors/vendors can post commercial content here with approval from admin. Please support our sponsors!
Topics: 329 Posts: 797
329 797
News about the latest advances and discoveries in sequencing and related technologies.
Topics: 163 Posts: 164
163 164
Core Facilities
Dealing with customer samples, data, and the challenges that come with both?
Topics: 127 Posts: 576
127 576
Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
Topics: 2,593 Posts: 7,793
2,593 7,793
by Polly
Webinar Series
Upcoming and on-demand webinars by SEQanswers and its partners.
Topics: 32 Posts: 47
32 47
Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
Topics: 41,653 Posts: 42,332
41,653 42,332
Sequencing Technologies/Companies
Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 50 Posts: 159
50 159
Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
Topics: 0 Posts: 0
0 0
Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
Topics: 3,034 Posts: 16,669
3,034 16,669
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
Topics: 391 Posts: 2,173
391 2,173
by KlausR
Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
Topics: 114 Posts: 591
Last Post: Flushing flow cells
114 591
by rlfr
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
Topics: 384 Posts: 2,175
384 2,175
Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
Topics: 0 Posts: 0
0 0
Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
Topics: 0 Posts: 0
0 0
Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 76 Posts: 275
Last Post: SeqStudio users?
76 275
Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
Topics: 79 Posts: 480
79 480
Archived Forums
A place for discussion of older forums and sequencing technologies.
Topics: 1,361 Posts: 7,278
Last Post: Rapid Libraries
1,361 7,278
by pmiguel
UK - Cambridge (37/67)
The Pipeline (81/587)
SOLiD (462/2,440)
454 Pyrosequencing (743/3,966)
Polonator (6/65)
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
Topics: 22,032 Posts: 102,980
22,032 102,980
Events / Conferences
Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)
Topics: 755 Posts: 1,263
755 1,263
Jobs Forums
Industry Jobs!
Job listings at companies that sponsor! Interested in posting a job? Contact admin@ for access.
Topics: 222 Posts: 253
222 253
Academic/Non-Profit Jobs
Post and look for academic, non-profit, or other non-industrial jobs here.
Topics: 1,601 Posts: 1,817
1,601 1,817
Applications Forums
Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,188 Posts: 9,866
2,188 9,866
Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
Topics: 6 Posts: 10
6 10
Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
Topics: 277 Posts: 990
277 990
De novo discovery
Wandering without a reference? Post here
Topics: 341 Posts: 1,700
341 1,700
Ever wonder what's growing in that hot spring or glacier?
Topics: 268 Posts: 847
268 847
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 356 Posts: 1,577
356 1,577
RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,859 Posts: 11,406
2,859 11,406
by gulsum
Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 42 Posts: 107
42 107
Personalized Genomics
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
Topics: 57 Posts: 290
57 290
by bione
Site News
Site Announcements
Announcements regarding SEQanswers
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52 201
Site Feedback/Suggestions
Please post all feedback or suggestions about the site in this forum.
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47 656
by Sbiljh1





Recent Articles


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    Spatial technologies have improved our understanding of the transcriptome by providing insights into the spatial organization and interactions of cells. While next-generation sequencing (NGS) remains an important approach and is the main focus of our community, its data alone is unable to resolve unique spatial information. Jacob Stern, Director of Product Management at 10X Genomics, emphasized how numerous researchers choose to use both spatial platforms and single-cell...
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