Bioinformatics Pipeline Development with Nextflow
How to manage your own data analysis pipelines using workflow management systems
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Dear all,
I hope you are doing well.
Firstly, I am sorry if my question looks naive, I am beginner in this field.
...09-30-2024, 08:36 AM -
Hi everyone,
I’m excited to join this community! I work as a clinical researcher, with a focus on using genomics and bioinformatics to...09-27-2024, 03:52 AM
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Introductions
New here? Stop in and introduce yourself. Where you are, what you work on, etc.
Topics: 2,276
Posts: 4,400
Last Post:
+1 on the forum
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2,276 | 4,400 |
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Sequencing Resources
SEQanswers sponsors/vendors can post commercial content here with approval from admin. Please support our sponsors!
Topics: 352
Posts: 820
Last Post:
WGBS vs RRBS
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News
News about the latest advances and discoveries in sequencing and related technologies.
Topics: 271
Posts: 273
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271 | 273 |
by seqadmin
Yesterday, 04:51 AM
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Core Facilities
Dealing with customer samples, data, and the challenges that come with both?
Topics: 127
Posts: 577
Last Post:
post-bisulphite conversion QC check
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General
Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
Topics: 2,611
Posts: 7,811
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Webinar Series
Upcoming and on-demand webinars by SEQanswers and its partners.
Topics: 39
Posts: 55
Last Post:
Single-Cell Multi-Omics for Disease Research
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Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
Topics: 41,653
Posts: 42,332
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41,653 | 42,332 |
by Biotechvana
03-27-2023, 11:17 PM
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Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 53
Posts: 164
Last Post:
G400 fastq header description?
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Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
Topics: 2
Posts: 5
Last Post:
Weird 16S custom primer issue on Aviti runs
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Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
Topics: 3,057
Posts: 16,723
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3,057 | 16,723 |
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Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
Topics: 392
Posts: 2,174
Last Post:
Ion Ampliseq library prep query
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392 | 2,174 |
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Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
Topics: 117
Posts: 596
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117 | 596 |
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Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
Topics: 385
Posts: 2,176
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385 | 2,176 |
by Osingh
02-20-2024, 05:21 AM
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Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
Topics: 0
Posts: 0
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Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
Topics: 0
Posts: 0
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 76
Posts: 278
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76 | 278 |
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Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
Topics: 79
Posts: 480
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79 | 480 |
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Archived Forums
A place for discussion of older forums and sequencing technologies.
Topics: 1,361
Posts: 7,278
Last Post:
Rapid Libraries
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Sub-Forums:
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Bioinformatics
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
Topics: 22,106
Posts: 103,071
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22,106 | 103,071 |
by duartemolha
09-26-2024, 06:58 AM
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Events / Conferences
Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)
Topics: 779
Posts: 1,292
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Industry Jobs!
Job listings at companies that sponsor SEQanswers.com! Interested in posting a job? Contact admin@ for access.
Topics: 222
Posts: 253
Last Post:
Senior Scientist, NGS
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Academic/Non-Profit Jobs
Post and look for academic, non-profit, or other non-industrial jobs here.
Topics: 1,603
Posts: 1,819
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1,603 | 1,819 |
01-08-2024, 03:56 AM
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| Applications Forums | ||||||||||||
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Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,200
Posts: 9,894
Last Post:
DNA library after size select has 2 peaks
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Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
Topics: 7
Posts: 14
Last Post:
Understanding UMAP
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
Topics: 278
Posts: 991
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De novo discovery
Wandering without a reference? Post here
Topics: 342
Posts: 1,701
Last Post:
Hifiasm and sex chromosomes from XY specimen
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342 | 1,701 |
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Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 273
Posts: 852
Last Post:
Impact of biomass on shotgun sequencing
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Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 357
Posts: 1,578
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357 | 1,578 |
12-09-2023, 09:17 PM
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RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,871
Posts: 11,424
Last Post:
Importing data from GEO to R for analysing
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Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 42
Posts: 107
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Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
Topics: 57
Posts: 290
Last Post:
Bione Genetic Testing for Cancer
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Site Announcements
Announcements regarding SEQanswers
Topics: 52
Posts: 201
Last Post:
Heading to ASHG 2023
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Site Feedback/Suggestions
Please post all feedback or suggestions about the site in this forum.
Topics: 48
Posts: 657
Last Post:
Making a table in BBCode does not work
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Dear all,
I hope you are doing well.
Firstly, I am sorry if my question looks naive, I am beginner in this field.
...09-30-2024, 08:36 AM -
Bioinformatics Pipeline Development with Nextflow
How to manage your own data analysis pipelines using workflow management systems
...Yesterday, 05:57 AM -
Hi everyone,
I’m excited to join this community! I work as a clinical researcher, with a focus on using genomics and bioinformatics to...09-27-2024, 03:52 AM -
Hello,
I am doing a comparison of duplicated reads on Illumina NovaSeq vs MGI G400 by checking the distribution of reads on the flowcell....09-25-2024, 02:37 AM
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Join us for an exciting webinar focused on innovative research in the field of single-cell multi-omics. Our expert...09-26-2024, 06:22 AM -
Single-cell studies are currently limited by expensive technologies or complex experimental workflows. Without the ability to profile...09-18-2024, 06:48 AM -
Join us for a webinar on the latest in pathogen genomics and epidemiology. Our experts will cover how NGS and machine learning can enhance understanding...08-26-2024, 09:56 AM
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Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...09-23-2024, 06:35 AM -
During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.
Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...09-09-2024, 10:59 AM -
The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...08-27-2024, 04:44 AM
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