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Channel: Events / Conferences
Yesterday, 12:59 AM -
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Use your standard DNA variant caller for SNP calling in Bisulfite-Seq data
Until now, specialised tools are required in order to access DNA...-
Channel: Bioinformatics
06-28-2022, 01:03 AM -
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by aliceseqCan someone please kindly give expertise on how I should go about pooling my samples. I have done library preparation for 80 samples INDIVIDUALLY (yes...
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Channel: Sample Prep / Library Generation
06-24-2022, 12:50 PM -
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Introductions
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Bacterial WGS on Ion Torrent
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understanding VCF ids
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syndip dataset for benchmark variant
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by pvaloblogs
06-26-2022, 08:43 AM
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Sequencing Technologies/Companies | |||
454 Pyrosequencing
Pyrosequencing in picotiter plates, custom arrays for enrichment/decomplexing. (Roche)
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Roche 454 RL adapters.. help!!!
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Service-based whole human genome sequencing with probe ligation chemistry.
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Helicos / Direct Genomics
True Single Molecule Sequencing, originally by Helicos, tech acquired by Direct Genomics
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Download of example Data Helicos
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Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Life Technologies)
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Oxford Nanopore
Single molecule sequencing via a protein nanopore, presently on the MinION.
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Priming Flongle Flowcell
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Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures
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"Open Source" instrument and sequencing by ligation chemistry (Danaher Motion-Dover)
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
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ABI 3130xl 50 cm Array NOS Lifetime
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regarding the values of quality
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List of next-next gen...
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Applications Forums | |||
Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
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by Zw1224
05-26-2022, 03:05 AM
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De novo discovery
Wandering without a reference? Post here
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by Rezaeir
08-16-2020, 08:43 AM
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Metagenomics
Ever wonder what's growing in that hot spring or glacier?
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Shallow shotgun metagenomics
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Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
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RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
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by scienceguy
06-09-2022, 11:28 AM
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Clinical Sequencing
Discuss issues unique to clinical sequencing.
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Posts: 102
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Regional/Local Communities | |||
Topics: 43
Posts: 70
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by narain
04-25-2022, 07:28 AM
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Personalized Genomics | |||
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
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Bione Genetic Testing for Cancer
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Hey Seq-ers!
Wanted to let you all know we at Edinburgh Genomics have a range of bioinformatics courses running this summer. From beginner...-
Channel: Events / Conferences
Yesterday, 12:59 AM -
-
by colinhercusHi,
I've downloaded some cram files for 30X GRCH38 alignments that I want to remap but I can't find any mention of which grch38.fa was used...-
Channel: Bioinformatics
06-21-2022, 04:07 AM -
-
Use your standard DNA variant caller for SNP calling in Bisulfite-Seq data
Until now, specialised tools are required in order to access DNA...-
Channel: Bioinformatics
06-28-2022, 01:03 AM -
-
by Rahel31Hi,
I am trying to switch from 16S to shotgun metagenomics, but as the number of samples is quite high (several hundreds of human stool samples),...-
Channel: Metagenomics
04-23-2022, 06:46 AM -
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by F.LazaroHello everyone,
Can someone explain why the NebNext Ultra DNA Lib Prep Kit is so expensive compared to the sum of the invidivual components?...-
Channel: Sample Prep / Library Generation
03-22-2018, 07:32 AM -