I'm thinking its guanidine...
-
Hi all, looking for some advice around RNA samples submitted to our core exhibiting additional broad peak from 200-1000bp.
I'm thinking its guanidine...06-02-2023, 04:07 AM -
Genome bioinformatics: from short- to long-read sequencing
A guide to the technology, analysis workflows, tools, and resources for next generation...05-31-2023, 05:07 AM -
Hi,
I would like to ask,
I have assembled chloroplast genome of cultivated species using Getorganelle, and got circular.
However...05-30-2023, 11:43 PM
No content found
Please log in to your account to view your subscribed posts.
| Directory | Topics | Posts | Last Post | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Introductions
New here? Stop in and introduce yourself. Where you are, what you work on, etc.
Topics: 2,252
Posts: 4,355
Last Post:
New Member
|
2,252 | 4,355 |
|
|||||||||
|
Sequencing Resources
SEQanswers sponsors/vendors can post commercial content here with approval from admin. Please support our sponsors!
Topics: 328
Posts: 796
|
328 | 796 |
|
|||||||||
|
News
News about the latest advances and discoveries in sequencing and related technologies.
Topics: 52
Posts: 53
|
52 | 53 |
by seqadmin
06-01-2023, 08:56 PM
|
|||||||||
|
General
Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
Topics: 2,583
Posts: 7,782
|
2,583 | 7,782 |
by GenomicSeq
05-15-2023, 07:35 AM
|
|||||||||
|
Core Facilities
Dealing with customer samples, data, and the challenges that come with both?
Topics: 127
Posts: 578
Last Post:
syndip dataset for benchmark variant
|
127 | 578 |
|
|||||||||
|
Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
Topics: 41,653
Posts: 42,332
|
41,653 | 42,332 |
by Biotechvana
03-27-2023, 11:17 PM
|
|||||||||
| Sequencing Technologies/Companies | ||||||||||||
|
Complete Genomics (MGI)
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 50
Posts: 158
Last Post:
Adapters sequence for trimming PE reads
|
50 | 158 |
|
|||||||||
|
Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
Topics: 0
Posts: 0
|
0 | 0 |
|
|||||||||
|
Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
Topics: 3,018
Posts: 16,629
Last Post:
Non-responsive over clustering issue
|
3,018 | 16,629 |
|
|||||||||
|
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
Topics: 391
Posts: 2,172
Last Post:
Analyzing IonTorrent data
|
391 | 2,172 |
|
|||||||||
|
Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
Topics: 109
Posts: 580
Last Post:
Priming Flongle Flowcell
|
109 | 580 |
|
|||||||||
|
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
Topics: 384
Posts: 2,175
Last Post:
July webinar: Cancer Transcriptomics
|
384 | 2,175 |
|
|||||||||
|
Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
Topics: 0
Posts: 0
|
0 | 0 |
|
|||||||||
|
Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
Topics: 0
Posts: 0
|
0 | 0 |
|
|||||||||
|
Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 76
Posts: 274
Last Post:
ABI 3130xl 50 cm Array NOS Lifetime
|
76 | 274 |
|
|||||||||
|
Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
Topics: 79
Posts: 481
|
79 | 481 |
|
|||||||||
|
Archived Forums
A place for discussion of older forums and sequencing technologies.
Topics: 1,361
Posts: 7,278
Last Post:
Rapid Libraries
|
1,361 | 7,278 |
|
|||||||||
|
Sub-Forums:
| ||||||||||||
| Bioinformatics | ||||||||||||
|
Bioinformatics
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
Topics: 21,993
Posts: 102,924
|
21,993 | 102,924 |
06-02-2023, 03:17 AM
|
|||||||||
|
Events / Conferences
Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)
Topics: 741
Posts: 1,254
|
741 | 1,254 |
by tj300542
06-02-2023, 12:07 PM
|
|||||||||
| Jobs Forums | ||||||||||||
|
Industry Jobs!
Job listings at companies that sponsor SEQanswers.com! Interested in posting a job? Contact admin@ for access.
Topics: 222
Posts: 253
Last Post:
Senior Scientist, NGS
|
222 | 253 |
|
|||||||||
|
Academic/Non-Profit Jobs
Post and look for academic, non-profit, or other non-industrial jobs here.
Topics: 1,595
Posts: 1,811
|
1,595 | 1,811 |
05-19-2023, 05:16 AM
|
|||||||||
| Applications Forums | ||||||||||||
|
Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,182
Posts: 9,848
Last Post:
Tapestation HS RNA - additional broad peak?
|
2,182 | 9,848 |
|
|||||||||
|
Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
Topics: 3
Posts: 7
Last Post:
PHLAT genotyping with scRNA-seq (10x)
|
3 | 7 |
|
|||||||||
|
Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
Topics: 277
Posts: 990
|
277 | 990 |
|
|||||||||
|
De novo discovery
Wandering without a reference? Post here
Topics: 341
Posts: 1,700
Last Post:
infering isoforms in merged transcriptome
|
341 | 1,700 |
|
|||||||||
|
Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 267
Posts: 840
|
267 | 840 |
by GenomicSeq
05-11-2023, 03:06 PM
|
|||||||||
|
Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 356
Posts: 1,577
Last Post:
DMRcaller Minimum Reads per Cytosine
|
356 | 1,577 |
|
|||||||||
|
RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,850
Posts: 11,391
|
2,850 | 11,391 |
by GenomicSeq
05-25-2023, 06:49 AM
|
|||||||||
|
Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 39
Posts: 104
|
39 | 104 |
by petch
11-13-2022, 09:42 AM
|
|||||||||
| Personalized Genomics | ||||||||||||
|
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
Topics: 57
Posts: 290
Last Post:
Bione Genetic Testing for Cancer
|
57 | 290 |
|
|||||||||
| Site News | ||||||||||||
|
Site Announcements
Announcements regarding SEQanswers
Topics: 51
Posts: 200
|
51 | 200 |
|
|||||||||
|
Site Feedback/Suggestions
Please post all feedback or suggestions about the site in this forum.
Topics: 47
Posts: 656
Last Post:
Cannot view attachments?
|
47 | 656 |
|
|||||||||
|
Webinar Series
Planning and feedback subforum for planning sequencing webinar episodes
Topics: 29
Posts: 44
|
29 | 44 |
|
|||||||||
-
Hi all, looking for some advice around RNA samples submitted to our core exhibiting additional broad peak from 200-1000bp.
I'm thinking its guanidine...06-02-2023, 04:07 AM -
7th Berlin Summer School in NGS Data Analysis
When? September 4-8, 2023
Where? Berlin, Germany
Link? Website
The
...05-07-2023, 11:46 PM -
RNA-Seq Data Analysis Workshop
Quality Control, Read Mapping, Visualization and Downstream Analyses
When? February 28 -...01-10-2022, 11:41 PM -
I'd like to introduce another member of the BBTools package, BBDuk. "Duk" stands for Decontamination Using Kmers. BBDuk is extremely fast,...04-24-2014, 03:00 PM
-
Contaminated DNA leads to lower-quality results, no matter what study you're doing. One of the most, if not the most, common contaminants in DNA sequencing...04-17-2014, 01:25 PM
-
Developments in sequencing technologies and methodologies have transformed the field of epigenetics, giving researchers a better way to understand the complex world of gene regulation and heritable modifications. This article explores some of the diverse sequencing methods employed in the study of epigenetics, ranging from classic techniques to cutting-edge innovations while providing a brief overview of their processes, applications, and advances.
Methylation Detect...05-31-2023, 10:46 AM -
Differential Expression and Data Visualization: Recommended Tools for Next-Level Sequencing Analysis
After covering QC and alignment tools in the first segment and variant analysis and genome assembly in the second segment, we’re wrapping up with a discussion about tools for differential gene expression analysis and data visualization. In this article, we include recommendations from the following experts: Dr. Mark Ziemann, Senior Lecturer in Biotechnology and Bioinformatics, Deakin University; Dr. Medhat Mahmoud Postdoctoral Research Fellow at Baylor College of Medicine;...05-23-2023, 12:26 PM -
Continuing from our previous article, we share variant analysis and genome assembly tools recommended by our experts Dr. Medhat Mahmoud, Postdoctoral Research Fellow at Baylor College of Medicine, and Dr. Ming "Tommy" Tang, Director of Computational Biology at Immunitas and author of From Cell Line to Command Line.
Variant detection and analysis tools
Mahmoud classifies variant detection work into two main groups: short variants (<50...05-19-2023, 10:03 AM
-
Join us for an insightful webinar, where we will dive into the bioinformatics process so you can learn how to analyze whole genome sequencing data. Our...05-30-2023, 01:22 PM
There are currently 1523 users online. 1 members and 1522 guests.
Most users ever online was 14,951 at 02:11 PM on 05-02-2022.
