I'm working in R and trying to demultiplex some data using cutadapt
I have three files:
barcodes.fastq.gz (which I have
...
-
Hi all,
I'm working in R and trying to demultiplex some data using cutadapt
I have three files:03-20-2023, 01:08 PM -
Hello everyone,
I am seeing two peaks (bimodal distribution) in my lib prep BioAnalyzer. The input sample is gDNA extracted from whole blood....You do not have permission to view this gallery.
This gallery has 2 photos.03-17-2023, 05:06 AM -
How does the Ovation RNA seq v2 kit generate double-stranded cDNA instead of single-stranded cDNA??!!
I am trying to use the Ovation...03-14-2023, 02:38 PM
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Hello everyone,
I am seeing two peaks (bimodal distribution) in my lib prep BioAnalyzer. The input sample is gDNA extracted from whole blood....You do not have permission to view this gallery.
This gallery has 2 photos.03-17-2023, 05:06 AM -
Hi all,
I'm working in R and trying to demultiplex some data using cutadapt
I have three files:barcodes.fastq.gz (which I have...03-20-2023, 01:08 PM -
How long is an ABI 3130xl 50 cm array if its never been opened and/or used? In other words, what's its shelf-life?01-25-2022, 01:39 PM
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We are a core facility that receives samples from multiple labs. We have one lab in particular that submits which are ran on a 3730XL. We are trying to...01-06-2023, 09:11 AM
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I wonder if anyone has information whether SeqStudio cathode buffer is the same as other running /cathode buffers for earlier ABI CE sequencers. In other...10-29-2018, 11:57 AM
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Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...Yesterday, 01:49 PM -
Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...03-10-2023, 05:31 AM -
Using automation to prepare sequencing libraries isn’t a new concept, and most researchers are aware that there are numerous benefits to automating this process. However, many labs are still hesitant to switch to automation and often believe that it’s not suitable for their lab. To combat these concerns, we’ll cover some of the key advantages, review the most important considerations, and get real-world advice from automation experts to remove any lingering anxieties....02-21-2023, 02:14 PM
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