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fchatonnet

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fchatonnet

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Last Activity: 11-27-2024, 12:28 AM

Joined: 09-04-2014

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fchatonnet replied to How to make a heatmap with the exons of a gene with bam file of RNA-seq in R-studio?

in RNA Sequencing

11-27-2024, 12:18 AM
Hi César,
it's not very clear how did you get from the bam files to the DGElist object, it seems that you used some kind of counts matrix, but...

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fchatonnet replied to DNA turns black over time - contamination?

in Sample Prep / Library Generation

11-27-2024, 12:11 AM
Hi AngelaT, this looks indeed like a fungal contamination.
What buffer do you use to elute your DNA? Water, Tris EDTA ?
It might be worth...

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fchatonnet replied to Primer Dimers in Library

in Sample Prep / Library Generation

11-13-2024, 01:21 AM
Dear seleenjaber,

a picture of an electrophoregram (BioAnalyzer analysis for example) would be nice to have an idea of your library size...

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fchatonnet replied to Base-by-Base Gene Coverage Analysis in .bam Files Using samtools

in Bioinformatics

11-13-2024, 01:13 AM
Hello ynkslzr,

some comments about your question: coverage is for me different from read depth in the way that it should be normalized by...

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