Hi all!
I have generated genome assemblies for my samples and would like to find the location of coding sequences in the contigs. Using coding...
-
Yesterday, 04:01 PM -
Next-generation sequencing (NGS) is the modern, second-revolution and the spearhead of an ever-accelerating field. The NGS technology performs innovative...Yesterday, 12:35 PM
-
Hi. I am new. I have Just graduated and have to start an internship. When I Will start I already have a task to perform and I would like to ask you what...07-24-2024, 01:18 AM
No content found
Please log in to your account to view your subscribed posts.
| Directory | Topics | Posts | Last Post | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Introductions
New here? Stop in and introduce yourself. Where you are, what you work on, etc.
Topics: 2,271
Posts: 4,391
|
2,271 | 4,391 |
by Bis
07-19-2024, 05:28 AM
|
|||||||||
|
Sequencing Resources
SEQanswers sponsors/vendors can post commercial content here with approval from admin. Please support our sponsors!
Topics: 339
Posts: 807
Last Post:
A Basic Guide to RNA-sequencing
|
339 | 807 |
|
|||||||||
|
News
News about the latest advances and discoveries in sequencing and related technologies.
Topics: 247
Posts: 249
|
247 | 249 |
|
|||||||||
|
Core Facilities
Dealing with customer samples, data, and the challenges that come with both?
Topics: 127
Posts: 577
Last Post:
post-bisulphite conversion QC check
|
127 | 577 |
|
|||||||||
|
General
Any topic/question that does not fit into the subcategories below. If you're unsure of where to put something, ask in here!
Topics: 2,606
Posts: 7,806
|
2,606 | 7,806 |
by Noof bk
06-07-2024, 12:23 AM
|
|||||||||
|
Webinar Series
Upcoming and on-demand webinars by SEQanswers and its partners.
Topics: 35
Posts: 51
Last Post:
Single Cell Genomics of Myeloid Malignancies
|
35 | 51 |
|
|||||||||
|
Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
Topics: 41,653
Posts: 42,332
|
41,653 | 42,332 |
by Biotechvana
03-27-2023, 11:17 PM
|
|||||||||
| Sequencing Technologies/Companies | ||||||||||||
|
Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 51
Posts: 160
Last Post:
Setting up and using MGI platforms
|
51 | 160 |
|
|||||||||
|
Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
Topics: 2
Posts: 5
Last Post:
Weird 16S custom primer issue on Aviti runs
|
2 | 5 |
|
|||||||||
|
Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
Topics: 3,054
Posts: 16,716
Last Post:
Adapter-dimer or low library diversity?
|
3,054 | 16,716 |
|
|||||||||
|
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
Topics: 392
Posts: 2,174
Last Post:
Ion Ampliseq library prep query
|
392 | 2,174 |
|
|||||||||
|
Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
Topics: 117
Posts: 596
|
117 | 596 |
|
|||||||||
|
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
Topics: 385
Posts: 2,176
|
385 | 2,176 |
by Osingh
02-20-2024, 05:21 AM
|
|||||||||
|
Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
Topics: 0
Posts: 0
|
0 | 0 |
|
|||||||||
|
Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
Topics: 0
Posts: 0
|
0 | 0 |
|
|||||||||
|
Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 76
Posts: 278
|
76 | 278 |
|
|||||||||
|
Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
Topics: 79
Posts: 480
|
79 | 480 |
|
|||||||||
|
Archived Forums
A place for discussion of older forums and sequencing technologies.
Topics: 1,361
Posts: 7,278
Last Post:
Rapid Libraries
|
1,361 | 7,278 |
|
|||||||||
|
Sub-Forums:
| ||||||||||||
| Bioinformatics | ||||||||||||
|
Bioinformatics
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
Topics: 22,088
Posts: 103,051
|
22,088 | 103,051 |
|
|||||||||
|
Events / Conferences
Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)
Topics: 774
Posts: 1,286
|
774 | 1,286 | ||||||||||
| Jobs Forums | ||||||||||||
|
Industry Jobs!
Job listings at companies that sponsor SEQanswers.com! Interested in posting a job? Contact admin@ for access.
Topics: 222
Posts: 253
Last Post:
Senior Scientist, NGS
|
222 | 253 |
|
|||||||||
|
Academic/Non-Profit Jobs
Post and look for academic, non-profit, or other non-industrial jobs here.
Topics: 1,603
Posts: 1,819
|
1,603 | 1,819 |
01-08-2024, 03:56 AM
|
|||||||||
| Applications Forums | ||||||||||||
|
Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,197
Posts: 9,889
Last Post:
Seeking Help: Abnormal ATAC-seq Results
|
2,197 | 9,889 |
|
|||||||||
|
Single Cell Sequencing
Techniques, discussions, and applications of single cell sequencing.
Topics: 6
Posts: 10
Last Post:
bam file missing barcode and UMI info
|
6 | 10 |
|
|||||||||
|
Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
Topics: 278
Posts: 991
|
278 | 991 |
|
|||||||||
|
De novo discovery
Wandering without a reference? Post here
Topics: 341
Posts: 1,700
Last Post:
infering isoforms in merged transcriptome
|
341 | 1,700 |
|
|||||||||
|
Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 273
Posts: 852
Last Post:
Impact of biomass on shotgun sequencing
|
273 | 852 |
|
|||||||||
|
Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 357
Posts: 1,578
|
357 | 1,578 |
12-09-2023, 09:17 PM
|
|||||||||
|
RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,869
Posts: 11,420
Last Post:
Experimental Design Violation?
|
2,869 | 11,420 |
|
|||||||||
|
Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 42
Posts: 107
|
42 | 107 |
|
|||||||||
| Personalized Genomics | ||||||||||||
|
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
Topics: 57
Posts: 290
Last Post:
Bione Genetic Testing for Cancer
|
57 | 290 |
|
|||||||||
| Site News | ||||||||||||
|
Site Announcements
Announcements regarding SEQanswers
Topics: 52
Posts: 201
Last Post:
Heading to ASHG 2023
|
52 | 201 |
|
|||||||||
|
Site Feedback/Suggestions
Please post all feedback or suggestions about the site in this forum.
Topics: 48
Posts: 657
Last Post:
Making a table in BBCode does not work
|
48 | 657 |
|
|||||||||
-
Hi all!
I have generated genome assemblies for my samples and would like to find the location of coding sequences in the contigs. Using coding...Yesterday, 04:01 PM -
Next-generation sequencing (NGS) is the modern, second-revolution and the spearhead of an ever-accelerating field. The NGS technology performs innovative...Yesterday, 12:35 PM
-
Hi. I am new. I have Just graduated and have to start an internship. When I Will start I already have a task to perform and I would like to ask you what...07-24-2024, 01:18 AM
-
Hello everyone!
I’m currently working on constructing a library for ATAC-seq with mouse intestinal tissue. I’ve prepared a 10μm thick...07-23-2024, 05:25 AM -
Hi all,
I ran a multiplex of 50 WGS libraries on Illumina NovaSeq system and the data output is low, approximately 40% undetermined reads....06-27-2024, 10:35 PM
widgetinstance 220 (Webinars) skipped due to lack of content & hide_module_if_empty option.
-
The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...07-08-2024, 03:19 PM -
In 2003, researchers from the Human Genome Project (HGP) announced the most comprehensive genome to date1. Although the genome wasn’t fully completed until nearly 20 years later2, numerous large-scale projects, such as the International HapMap Project and 1000 Genomes Project, continued the HGP's work, capturing extensive variation and genomic diversity within humans. Recently, newer initiatives have significantly increased in scale and expanded beyond genomics, offering a more detailed...06-25-2024, 06:43 AM -
While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...06-06-2024, 07:15 AM