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Introductions
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seqadmin
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News about the latest advances and discoveries in sequencing and related technologies.
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firehurtnova
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Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
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Sequencing Technologies/Companies
Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
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Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
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Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
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3,030 16,658
albert liu
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
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391 2,172
Ben3
by Ben3
Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
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111 585
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
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384 2,175
Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
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Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
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Last Post: SeqStudio users?
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hamzairshad267
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Metagenomics
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Epigenetics
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Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
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Clinical Sequencing
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Personalized Genomics
Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
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Sbiljh1
by Sbiljh1

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  • seqadmin
    How RNA-Seq is Transforming Cancer Studies



    Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...
    09-07-2023, 11:15 PM
  • seqadmin
    Methods for Investigating the Transcriptome




    Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.

    Whole Transcriptome RNA-seq
    Whole transcriptome sequencing...
    08-31-2023, 11:07 AM
  • seqadmin
    Modern Methods for Phased Genomes


    Continual advancements in genomic and computational technologies have allowed researchers to construct precise pictures of individual genomes and identify variations that distinguish one genome from another. In order to fully understand the impact of these variants, it is essential to determine their chromosomal context in a process known as phasing. In this article, we'll explore some of the current technologies and approaches fundamental to this process.

    What is ph...
    08-18-2023, 07:51 AM

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