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Hi! I'm Alisa and I'm currently working as a content writer. I focus on developing my writing skills on technology topics such as big data, data analytics,...Yesterday, 07:43 PM -
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I'm currently preparing library preps using a Tn5 enzyme. One day last week, library prep worked (although the fragment sizes...Yesterday, 08:10 AM -
Hi, I am planning to check the difference of gene expression (including coding and non-coding genes) for RNA of a time course during induced differentiation...08-16-2022, 10:18 PM
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Introductions
New here? Stop in and introduce yourself. Where you are, what you work on, etc.
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Last Post:
Hi everyone!
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General
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Posts: 7,766
Last Post:
FastQ/BAM compression
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2,564 | 7,766 |
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Core Facilities
Dealing with customer samples, data, and the challenges that come with both?
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Posts: 579
Last Post:
syndip dataset for benchmark variant
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128 | 579 |
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Events / Conferences
Relevant events or scientific conferences that may be of interest to the community. (Mgmt reserves right to limit commercial content without approval.)
Topics: 712
Posts: 1,214
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712 | 1,214 |
by barrytmock
08-17-2022, 01:11 AM
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Literature Watch
Discussion of any scientific study related to high content or next generation genomics. Whole genome association, metagenomics, digital gene expression, etc.
Topics: 41,772
Posts: 42,461
Last Post:
Mentions of SEQanswers in the Literature
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41,772 | 42,461 |
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| Sequencing Technologies/Companies | |||
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454 Pyrosequencing
Pyrosequencing in picotiter plates, custom arrays for enrichment/decomplexing. (Roche)
Topics: 762
Posts: 3,982
Last Post:
Roche 454 RL adapters.. help!!!
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762 | 3,982 |
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MGISEQ (FKA Complete Genomics)
Service-based whole human genome sequencing with probe ligation chemistry.
Topics: 50
Posts: 161
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50 | 161 |
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Helicos / Direct Genomics
True Single Molecule Sequencing, originally by Helicos, tech acquired by Direct Genomics
Topics: 29
Posts: 155
Last Post:
Сollapse of Helicos
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29 | 155 |
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Illumina/Solexa
Bridged amplification & clustering followed by sequencing by synthesis. (Genome Analyzer / HiSeq / MiSeq)
Topics: 2,983
Posts: 16,517
Last Post:
Pooling of amplicon libraries for MiSeq
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2,983 | 16,517 |
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Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Life Technologies)
Topics: 388
Posts: 2,217
Last Post:
Barcode and Adapter Sequences
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388 | 2,217 |
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Oxford Nanopore
Single molecule sequencing via a protein nanopore, presently on the MinION.
Topics: 105
Posts: 565
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105 | 565 |
by afkoeppel
Yesterday, 02:57 AM
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Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures
Topics: 383
Posts: 2,173
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383 | 2,173 |
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Polonator
"Open Source" instrument and sequencing by ligation chemistry (Danaher Motion-Dover)
Topics: 5
Posts: 70
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5 | 70 |
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 75
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Last Post:
ABI 3130xl 50 cm Array NOS Lifetime
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75 | 266 |
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SOLiD
Sequencing by Oligo Ligation/Detection (Life Technologies)
Topics: 462
Posts: 2,443
Last Post:
regarding the values of quality
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462 | 2,443 |
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The Pipeline
Sequencing technologies without a commercially released platform (Oxford Nanopore, Halcyon Molecular, etc.)
Topics: 79
Posts: 577
Last Post:
List of next-next gen...
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79 | 577 |
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Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
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Posts: 472
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75 | 472 |
08-15-2022, 08:14 AM
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Vendor Forum
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Topics: 329
Posts: 806
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329 | 806 |
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| Bioinformatics | |||
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Bioinformatics
Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
Topics: 21,843
Posts: 102,609
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21,843 | 102,609 |
by Alisa9
Yesterday, 08:34 PM
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Open Genomics Engine Project
Open discussion forum for Open Genomics Engine contributors and users
Topics: 9
Posts: 40
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9 | 40 |
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| Jobs Forums | |||
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Industry Jobs!
Job listings at companies that sponsor SEQanswers.com! Interested in posting a job? Contact [email protected] for access.
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Senior Scientist, NGS
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215 | 246 |
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Academic/Non-Profit Jobs
Post and look for academic, non-profit, or other non-industrial jobs here.
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Posts: 1,767
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1,541 | 1,767 |
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| Applications Forums | |||
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Sample Prep / Library Generation
Techniques and protocol discussions on sample preparation, library generation, methods and ideas
Topics: 2,152
Posts: 9,747
Last Post:
Tagmentation library prep is not working!
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2,152 | 9,747 |
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Genomic Resequencing
Variant discovery in previously sequenced genomes/regions
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277 | 988 |
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De novo discovery
Wandering without a reference? Post here
Topics: 340
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Last Post:
Can't get Ray working
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340 | 1,713 |
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Metagenomics
Ever wonder what's growing in that hot spring or glacier?
Topics: 261
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Last Post:
Shallow shotgun metagenomics
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261 | 829 |
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Epigenetics
Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.
Topics: 353
Posts: 1,572
Last Post:
Minimum Criteria to Publish ChIP-seq data
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353 | 1,572 |
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RNA Sequencing
Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
Topics: 2,839
Posts: 11,356
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2,839 | 11,356 |
by greekkey
08-16-2022, 10:18 PM
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Clinical Sequencing
Discuss issues unique to clinical sequencing.
Topics: 37
Posts: 102
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37 | 102 |
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| Regional/Local Communities | |||
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Topics: 43
Posts: 70
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43 | 70 |
by narain
04-25-2022, 07:28 AM
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| Personalized Genomics | |||
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Personalized Genomics
Efforts to understand one's own genome. Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.
Topics: 58
Posts: 294
Last Post:
Bione Genetic Testing for Cancer
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58 | 294 |
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| Site News | |||
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Site Announcements
Announcements regarding SEQanswers
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Posts: 198
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46 | 198 |
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Site Feedback/Suggestions
Please post all feedback or suggestions about the site in this forum.
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Last Post:
Cannot view attachments?
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48 | 661 |
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Wiki Discussion
Discussions about SEQwiki. Problems, suggestions, offers to help...
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Last Post:
Best Skip Bin Hire Beenleigh Services
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34 | 275 |
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Webinar Series
Planning and feedback subforum for planning sequencing webinar episodes
Topics: 25
Posts: 41
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25 | 41 |
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Hi! I'm Alisa and I'm currently working as a content writer. I focus on developing my writing skills on technology topics such as big data, data analytics,...Yesterday, 07:43 PM
-
Hello all,
I'm currently preparing library preps using a Tn5 enzyme. One day last week, library prep worked (although the fragment sizes...Yesterday, 08:10 AM -
We are performing MinION sequencing of DNA extracted from fresh water samples.
We got some preliminary results that suggested possible contamination,...02-02-2022, 07:32 AM -
Hi, I am planning to check the difference of gene expression (including coding and non-coding genes) for RNA of a time course during induced differentiation...08-16-2022, 10:18 PM
-
Hi, I am planning to analyzing human WGS data with the T2T-CHM13 genome as reference, which is now regarded as the complete sequence of a human genom...08-16-2022, 08:53 PM