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seqadmin
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Sequencing Technologies/Companies
Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
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Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
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Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
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Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
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Single-molecule sequencing via a protein nanopore.
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Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
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Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
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Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
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Automated dye terminator ("Sanger") chemistries
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Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
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Discuss issues unique to clinical sequencing.
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Personalized Genomics
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  • seqadmin
    Choosing Between NGS and qPCR



    Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...
    10-18-2024, 07:11 AM
  • seqadmin
    Non-Coding RNA Research and Technologies




    Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.

    Nobel Prize for MicroRNA Discovery
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    10-07-2024, 08:07 AM
  • seqadmin
    Recent Developments in Metagenomics





    Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...
    09-23-2024, 06:35 AM

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