Hej
I am working on an Illumina paired-end Amplicon workflow. Now I need to align my unique reads in order to produce a phylogeny what...
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Best Aligner for Duplicated Sequences
I'm looking around for some input from fellow SEQers. I'm experimenting with different aligners. I'm looking for an aligner that does a good job of correctly...
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Introducing BBSplit: Read Binning Tool for Metagenomes and Contaminated Libraries
BBSplit is a tool that bins reads by mapping to multiple references simultaneously, using BBMap. The reads go to the bin of the reference they map to...Last edited by Brian Bushnell; 09-16-2014, 08:29 AM.
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Which Sequence aligners to use?
Hello wise people,
I'm trying to align a sequence of a gene that is present in two related species. Each of the genes in either species...
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A can of Worms - Which all purpose aligner to replace BWA 0.5.7 for Illumina data?
Hi all,
Currently in our pipeline we are using BWA 0.5.7 in at least a part of all types of illumina data analysis.
By all...
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Greetings from Real Time Genomics
Hoping to answer questions from the community on the use of Real Time Genomics software, RTG Investigator 2.2. Please download at www.realtimegenomic...
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Mitochondrial Genome Assembly
Hi all,
I'm new to the bioinformatics world and am looking for an assembly/alignment program that would work well for the mammalian mitochondrial...
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BOAT aligner output format converter?
Hi all,
I am using the BOAT aligner (quite effectively) but I need to get the output to SAMtools format somehow. I was wondering if there...
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Very Short Read aligner
Hi All,
I am in process of building my own short read aligner for a lab working with cancer-genome sequencing. And have following questions:...
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BWA Alignment Segmentation Fault
I'm trying to align a set of 51bp paired-end Illumina reads from a human cell line cDNA library. BWA does a great job aligning my reads to the indexed...