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Complete Genomics (MGI)
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 50
Posts: 158
Last Post:
Adapters sequence for trimming PE reads
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50 | 158 |
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Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
Topics: 0
Posts: 0
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0 | 0 |
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Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
Topics: 3,018
Posts: 16,629
Last Post:
Non-responsive over clustering issue
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3,018 | 16,629 |
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Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
Topics: 391
Posts: 2,172
Last Post:
Analyzing IonTorrent data
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391 | 2,172 |
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Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
Topics: 109
Posts: 580
Last Post:
Priming Flongle Flowcell
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109 | 580 |
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Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
Topics: 384
Posts: 2,175
Last Post:
July webinar: Cancer Transcriptomics
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384 | 2,175 |
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Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
Topics: 0
Posts: 0
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0 | 0 |
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Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
Topics: 0
Posts: 0
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0 | 0 |
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Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 76
Posts: 274
Last Post:
ABI 3130xl 50 cm Array NOS Lifetime
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76 | 274 |
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Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
Topics: 79
Posts: 481
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79 | 481 |
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Archived Forums
A place for discussion of older forums and sequencing technologies.
Topics: 1,361
Posts: 7,278
Last Post:
Rapid Libraries
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1,361 | 7,278 |
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Developments in sequencing technologies and methodologies have transformed the field of epigenetics, giving researchers a better way to understand the complex world of gene regulation and heritable modifications. This article explores some of the diverse sequencing methods employed in the study of epigenetics, ranging from classic techniques to cutting-edge innovations while providing a brief overview of their processes, applications, and advances.
Methylation Detect...Yesterday, 10:46 AM -
Differential Expression and Data Visualization: Recommended Tools for Next-Level Sequencing Analysis
After covering QC and alignment tools in the first segment and variant analysis and genome assembly in the second segment, we’re wrapping up with a discussion about tools for differential gene expression analysis and data visualization. In this article, we include recommendations from the following experts: Dr. Mark Ziemann, Senior Lecturer in Biotechnology and Bioinformatics, Deakin University; Dr. Medhat Mahmoud Postdoctoral Research Fellow at Baylor College of Medicine;...05-23-2023, 12:26 PM -
Continuing from our previous article, we share variant analysis and genome assembly tools recommended by our experts Dr. Medhat Mahmoud, Postdoctoral Research Fellow at Baylor College of Medicine, and Dr. Ming "Tommy" Tang, Director of Computational Biology at Immunitas and author of From Cell Line to Command Line.
Variant detection and analysis tools
Mahmoud classifies variant detection work into two main groups: short variants (<50...05-19-2023, 10:03 AM