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Platform specific questions, news, discussions

Forums Topics Posts Last Post
Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 52 Posts: 161
Last Post: WGS vs WES
52 161
boldizsara
Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
Topics: 2 Posts: 5
2 5
Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
Topics: 3,057 Posts: 16,723
3,057 16,723
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
Topics: 392 Posts: 2,174
392 2,174
Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
Topics: 117 Posts: 596
117 596
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
Topics: 385 Posts: 2,176
385 2,176
Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
Topics: 0 Posts: 0
0 0
Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
Topics: 0 Posts: 0
0 0
Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 76 Posts: 278
76 278
Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
Topics: 79 Posts: 480
79 480
Archived Forums
A place for discussion of older forums and sequencing technologies.
Topics: 1,361 Posts: 7,278
Last Post: Rapid Libraries
1,361 7,278
pmiguel
by pmiguel
Sub-Forums:
UK - Cambridge (37/67)
The Pipeline (81/587)
SOLiD (462/2,440)
454 Pyrosequencing (743/3,966)
Polonator (6/65)

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  • seqadmin
    Addressing Off-Target Effects in CRISPR Technologies
    by seqadmin






    The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...
    08-27-2024, 04:44 AM
  • seqadmin
    Selecting and Optimizing mRNA Library Preparations
    by seqadmin



    Sequencing mRNA provides a snapshot of cellular activity, allowing researchers to study the dynamics of cellular processes, compare gene expression across different tissue types, and gain insights into the mechanisms of complex diseases. “mRNA’s central role in the dogma of molecular biology makes it a logical and relevant focus for transcriptomic studies,” stated Sebastian Aguilar Pierlé, Ph.D., Application Development Lead at Inorevia. “One of the major hurdles for...
    08-07-2024, 12:11 PM

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