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Platform specific questions, news, discussions

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Illumina/Solexa
Bridged amplification & clustering followed by sequencing by synthesis. (Genome Analyzer / HiSeq / MiSeq)
Topics: 3,007 Posts: 16,582
3,007 16,582
Ben3
by Ben3
Oxford Nanopore
Single molecule sequencing via a protein nanopore, presently on the MinION.
Topics: 106 Posts: 571
106 571
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures
Topics: 384 Posts: 2,175
384 2,175
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Life Technologies)
Topics: 390 Posts: 2,171
390 2,171
MGISEQ (FKA Complete Genomics)
Service-based whole human genome sequencing with probe ligation chemistry.
Topics: 50 Posts: 158
50 158
Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 76 Posts: 270
76 270
jblain_3
Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
Topics: 79 Posts: 481
79 481
Sequencing Resources
SEQanswers sponsors/vendors can post commercial content here with approval from admin. Please support our sponsors!
Topics: 337 Posts: 807
337 807
Archived Forums
A place for discussion of older forums and sequencing technologies.
Topics: 1,361 Posts: 7,278
Last Post: Rapid Libraries
1,361 7,278
pmiguel
by pmiguel
Sub-Forums:
UK - Cambridge (37/67)
The Pipeline (81/587)
SOLiD (462/2,440)
454 Pyrosequencing (743/3,966)
Polonator (6/65)

News

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Recent Articles

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  • seqadmin
    A Brief Overview and Common Challenges in Single-cell Sequencing Analysis
    by seqadmin


    ​​​​​​The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i...

    01-24-2023, 01:19 PM
  • seqadmin
    Introduction to Single-Cell Sequencing
    by seqadmin
    Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.

    The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes
    ...
    01-09-2023, 03:10 PM
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