| Forums | Topics | Posts | Last Post | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Complete Genomics
DNA single-strand circularization followed by rolling circle amplification (RCA) to form DNA nanoballs for DNBSEQ.
Topics: 51
Posts: 160
Last Post:
Setting up and using MGI platforms
|
51 | 160 |
|
|||||||||
|
Element Biosciences
Avidity sequencing - multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.
Topics: 2
Posts: 5
Last Post:
Weird 16S custom primer issue on Aviti runs
|
2 | 5 |
|
|||||||||
|
Illumina (Solexa)
Bridged amplification & clustering followed by sequencing by synthesis.
Topics: 3,054
Posts: 16,716
Last Post:
Adapter-dimer or low library diversity?
|
3,054 | 16,716 |
|
|||||||||
|
Ion Torrent
Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)
Topics: 392
Posts: 2,174
Last Post:
Ion Ampliseq library prep query
|
392 | 2,174 |
|
|||||||||
|
Oxford Nanopore
Single-molecule sequencing via a protein nanopore.
Topics: 117
Posts: 596
|
117 | 596 |
|
|||||||||
|
Pacific Biosciences
Single-molecule real-time observation of DNA polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)
Topics: 385
Posts: 2,176
|
385 | 2,176 |
by Osingh
02-20-2024, 05:21 AM
|
|||||||||
|
Singular Genomics
Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.
Topics: 0
Posts: 0
|
0 | 0 |
|
|||||||||
|
Ultima Genomics
Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.
Topics: 0
Posts: 0
|
0 | 0 |
|
|||||||||
|
Sanger/Dye Terminator
Automated dye terminator ("Sanger") chemistries
Topics: 76
Posts: 278
|
76 | 278 |
|
|||||||||
|
Service Providers
Discussions about next gen sequencing service providers, reviews & experiences, etc. No overtly commercial content permitted without prior approval.
Topics: 79
Posts: 480
|
79 | 480 |
|
|||||||||
|
Archived Forums
A place for discussion of older forums and sequencing technologies.
Topics: 1,361
Posts: 7,278
Last Post:
Rapid Libraries
|
1,361 | 7,278 |
|
|||||||||
|
Sub-Forums:
| ||||||||||||
widgetinstance 229 (Webinars) skipped due to lack of content & hide_module_if_empty option.
-
The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...07-08-2024, 03:19 PM -
In 2003, researchers from the Human Genome Project (HGP) announced the most comprehensive genome to date1. Although the genome wasn’t fully completed until nearly 20 years later2, numerous large-scale projects, such as the International HapMap Project and 1000 Genomes Project, continued the HGP's work, capturing extensive variation and genomic diversity within humans. Recently, newer initiatives have significantly increased in scale and expanded beyond genomics, offering a more detailed...06-25-2024, 06:43 AM -
While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...06-06-2024, 07:15 AM