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post-bisulphite conversion QC check
Does anyone know of a quicker alternative to PCR/agarose gel when QCing bisulphite conversion in large numbers of samples?
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quality check 1 ng DNA
Hi everybody
I am willing to sequence with MiSeq using a Nextera XT library starting with very challenging samples of not cultivable bacteria....
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FastQC results for 16s reads
Hi
I have some FastQC results I have generated from Illumina 16s sequences. I have attached an example of the results for one of the demultiplexed...
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BBSplit result statistics
I use bbsplit.sh to run my analysis as following:
...Code:./bbmap/bbsplit.sh in1=R1_001_bc_prefix.fastq.gz in2=R2_001_bc_prefix.fastq.gz ref=hg19.fa,mm10.fa
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Using trimmomatic on multiple paired-end read files
I need help to write a for loop to run Trimmomatic tool for quality trimming of paired end fastq files.
I need to write a for loop so that I can...Last edited by shashankgupta; 02-14-2017, 04:49 AM.
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AlignQC - Long-read alignment quality reporting software
Hello all, I haven't started many threads so please redirect me to the right place if I'm not supposed to self-promote here, or there is a tools introduction...
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Minimum contig coverage to keep
Dear all,
I am working on de novo assembly of bacterial genomes. Genomes were sequenced with Miseq 2x250 libs with an average coverage...
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ATAC-seq quality control
Hello,
I am analysing ATAC-seq data for the first time and am wondering what typical values are for:
Total number of reads...
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Quality check with barcordes
Hello guys!
I'm really beginner at bioinformatics.
I work with viral metagenomics and my library is going to be constructed with Nextera...
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ChIP-seq library pre-processing
Hello everyone,
I am really hoping to find some ChIP-seq seasoned analysers around
I have several questions...
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Duplication levels
Hello all,
I'm currently analyzing a single-end 50bp-read RNAseq data, which was sequenced at an outside facility. I've got a very naive question,...
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Mate pair sequencing - quality, duplication, throughput
We recently got Illumina mate pair sequencing data for a human sample. I am trying to do some some Quality Check to see how good the data is.
...