66 results in 0.0025 seconds.
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eland extended
I've been given some 'eland extended' files to work with. There really isn't much online about these. I'm trying to convert them to sam/bam format or... -
TruSeq Custom Amplicon
Hi All,
I wonder if anyone can point me to a publication using Illumina TruSeq Custom Amplicon, with kind of in-depth description of the... -
Running mate pair samples on MiSeq
Hi All,
I am fairly certain that what I am going to ask might have been asked before so forgive me for my ignorance. We are trying to run... -
Solexa reads contamination
Hallo to all,
analysing the reads obtained from a Solexa sequencing of a library of small RNAs I obtained a sequence overrepresented (according... -
G'day! New on NGS
Hi all,
I'm Paul from Sydney. I am currently working on getting iCLIP protocol to work on my protein of interest. So far I am on the right... -
Problem with hybrid assebly with Celera Assembly
Hello, everyone,
Celera Assembler(CA) 6.1 can do hybrid assembly now.
I have 3 types of reads, Solexa (100bp read length, 500bp... -
RNA-seq multiplexing
I'm currently planning my first large RNA-seq experiment and was unsure about large-scale multiplexing.
I will have a total of 48 libraries... -
About PF definition in qseq file
Hi, all:
as we know, the last column in qseq file named PF, and who can give me the complete definition of PF?
Thank you!... -
unrecognized .txt format
Hi,
I recently received data from illumina, and I am unable to determine the file format or how to convert it to a fastq for bwa. I found... -
Whole-genome amplification prior to Illumina sequencing?
Hello,
Does anyone have any experience sequencing samples on the Illumina platform that have been whole-genome amplified? Has anyone explicitly... -
Velvet insert length on Illumina NGS Paired end reads
Hello,
I am new to Velvet and I am trying to run it on my llumina NGS Paired end reads (I know that I have to shuffle them).
The only... -
how to use bwa? and how to set paremeters for 75bp reads
Hello everybody.
i'm a new user of bwa to align solexa reads to the reference genome hg19.i have finished bwa index for the hg19 genome,but... -
Bowtie Paired end 100% failed to align
Hello all,
I am trying to use Bowtie to align paired end sequencing data from solexa, but I am consistently getting
... -
behaviour of bwa mapping for homologous regions.
Hi
I am using bwa on paired end illumina reads for identifying inter chromosomal translocation.
I have used breakdancer on the data but... -
Augustus with RNA-Seq
Hey guys, I'm new with bioinformatics and I'm currently working with gene prediction. I would like to know if has someone here that ever use Augustus...
