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Drerio · 02-14-2012, 11:05 AM

Hi All -

I am new to the forum, but I am hoping that you might be able too help me parse a question of library preparation. I am about...

ZhengXia · 11-26-2011, 09:50 AM

Hi all,
We have a single end strand specific RNA-seq using dUTP protocol. Those reads (single end) from this protocol should be mapped to sense...

frymor · 07-27-2011, 10:47 PM

Hi,

I am trying to create strand-specific wig files from a bam file.
I used the pileup option and i thought it worked very good....

ewilbanks · 05-10-2011, 08:06 PM

Hi all,

Does anyone know of a fast, handy way to calculate coverage of the forward and reverse strands in a genome independently?
...

woolfman · 02-23-2011, 07:49 AM

Hi,

does anyone know a tool or program that can create a density profiles across the genome (I assume bedGraph would be the best format)...

David Harmin · 02-16-2011, 09:34 AM

Hello, everyone, I'm new here.

I usually analyze unpaired-read data from SOLiD sequencing of RNA-Seq libraries. The output from mapping...

ghbore · 11-22-2010, 07:13 AM

Dear All,
I am trying to map strand-specific pair-end reads using Tophat v1.1.4, but in the result BAM file 'ISIZE' or 'TLEN' field is identical...

RNA_seq on (non-model) less well annotated genomes