Course: Comparative Genomics (2nd edition)
Where: Free University Berlin (Germany)
When: 7-11 October 2019...
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Which PacBio ouputs use to do novo assembly?
Hello, I was given all the outputs of the result of a whole genome sequencing of a bacteria, and there is a lot of them and I don’t understand which...
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dacotahm started a topic Can you recommend a commercial HMW gDNA extraction service for insects in the US?in GeneralCan you recommend a commercial HMW gDNA extraction service for insects in the US?
Hello,
I'm working on some extractions but am under a time crunch. I'd like to send away for extractions as a plan-B. Can anyone recommend...
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Alternative to Qiagen Puregene Tissue kit for HMW gDNA?
Hello,
We're extracting high molecular weight gDNA for PacBio from an insect-a bee- using the Qiagen Puregene Tissue kit (4g). We have...
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PacBio consensus quality
Hello
I have sequenced a BAC clone with PacBio RSII
To make the assembly I used Facon through pbbioconda and for polishing I used quiver...Last edited by phleroy; 01-25-2019, 01:07 AM.
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Aligner for PacBio RNASeq
I am wondering if I can use BLASR for aligning RNASeq reads from Human cell lines. I am interested in identifying the splice sites. I read this paper...
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Targeted sequencing workflow?
I have a non-barcoded multiple samples pool of long amplicons sequenced on RSII. I'm trying to figure out the correct workflow. My goal is to perform...
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Course: Comparative Genomics
Course: Comparative Genomics
General Topic: Comparative genomics with a focus on structural variation
When: 1-5 October...
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Question on multiplexing - long reads
Hi All
So I have 2-3 number of 2MB bacterial genome and two plasmids of 17 kb each length. I am looking at the feasilbility of multiplexing...
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Microbial SMRT Grant Program - Win Sequencing on the Sequel System - Enter by 7/20
We're pleased to announce the 2018 Microbial Genomics SMRT Grant Program! Move beyond draft genomes with PacBio microbial whole genome sequencing. Submit...
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pacbio sequel system
how much do core facilities charge to run the instrument, not per sample or SMRTcell but just to recover the cost of running the device? (electricity,...
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Ploidy estimation or decision using pacbio sequel data
Hi all.
After finish assemble fungus genome from pacbio sequel using SMRT link or Falcon assembler, the genome size is twice as much as I...Last edited by yjpark1091; 04-22-2018, 11:01 PM.
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PacBio small genome sequencing from SNPsaurus
SNPsaurus is pleased to announce the launch of PacBio small genome sequencing as a service offering. We offer library prep, PacBio Sequel sequencing,...
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PacBio raw .bam file
I have just received data from my first PacBio sequencing operation and was unaware that the new output format was in .bam file for raw reads, as they...
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problem with samstat on PacBio alignment
Dear all,
I ran an alignment of Sequel data using blasr. Now I want to get some metrics about the sam file using samstat 1.5.1 (running on Ubuntu...