Hello,
I have paired-end 100bp reads generated from BGI-seq 500. The sequencing center did some adapter removal trimming before delivering...
25 results in 0.0023 seconds.
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Passed FastQC - but adapters still present?
Hi there - I am brand new to both Bioinformatics and this forum so I hope I'm posting in the right place; and that my questions make sense!
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Trimmomatic: Not trimming..
Hey! I am trying to use Trimmomatic to trim Illumina adapters sequences from my TruSeq Small RNA library SE 75bp reads off of a HiSeq but I am unsuccessful......Last edited by lchippy; 11-12-2017, 11:34 AM. -
rRNA and adapter removal bondaries in RNA-seq HiSeq 3000 Illumina
Hello
I am new in RNA-seq analysis and it is difficult for me to find out info about quality assessment in the matter. I did an analysis... -
Ion Torrent adapter artefacts
Hi guys!
We recently began working with Ion Torrent PGM. At first we were thinking that server cuts off all adapters and barcodes and we... -
Illumina paired end adapter contamination problem
Hello everyone!
I have rna-seq Illumina paired end reads and want to proceed with adapter trimming.
I have some confusions:
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Trimming adapter sequences - is it really necessary?
Removal of adapter sequences in a process called read trimming, or clipping, is one of the first steps in analyzing NGS data. With more than 30 published... -
Trimmomatic paired end- dropped reverse reads
Hi,
I am working on a re-sequencing project and have sequenced some whole genomes using Illumina HiSeq 2000 (150 bp paired end reads), which I... -
Method for Identifying Adapters??
I have been given Illumina paired end sequences for a chicken genome. I do not have access to the experiment protocol and the adapter files nor do I...Last edited by nemis00; 10-17-2015, 05:32 PM. -
processing Fastq files from HiSeq2000 single end and RNAseq analysis
Hello all,
i apologise in advance for asking some basic questions
i have recently started working on RNA-Seq. i have fastq... -
About adapter trimming
Hi, everyone. I have a question regarding the adapter trimming of reads generated by Miseq. I am doing RNA seq using the Illumina RNA sample prep kit... -
Please help my BBMap cannot remove Illumina adapter
Dear great helpers,
I'm an RNAseq starter and trying to find a good tool for fastq file preprocessing. I currently use BBmap to trim Illumina... -
Appropriate Adapter Sequence fora trimming? RNAseq Transcriptome
For previous studies I have contracted out both Illumina sequencing and RNAseq analysis of transcriptomes. An updated reference genome was added to NCBI... -
Trimmomatic and Multiplexed Samples
Hello and thanks for reading,
I'm performing adapter trimming for the first time using Trimmomatic on paired-end reads. We solicited a... -
Automatic adapter identification for trimming
Hello everybody,
I am kind of new ot the analysis of NGS data, so I am sorry if my questions
my be to simple, or not easy to understand...
