Hello everyone,
Kindly help me how to find a virulent strain of a virus by aligning the sequences in the databases.
Thank you...
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What tool can align a ton of NGS data from a large region to a tiny reference?
Hi all,
I have NGS data from a patient: Genomic DNA fragments ranging from 300-600bp sequenced on the MiSeq (2x250bp reads) prepared with...
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D. Melanogaster ChIP
I recently tried to do a ChIP-like experiment on chromatin from drosophila S2 cells and found that a large portion of my ChIP reads, but also my input...
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Which Sequence aligners to use?
Hello wise people,
I'm trying to align a sequence of a gene that is present in two related species. Each of the genes in either species...
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Getting just a few alignments with Tophat2
I made an alignment for a 1.1GB file, the RNA-seq reads were in fastq (Illumina) format. The reference was in fasta, so i decided to convert the large...
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Ugene
I am trying to align my 454 sequences to reference sanger sequences in order to see the amount of intra-individual virion diversity. I'm working with...
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bwa alignement outside of the reference sequence
Hi,
Using bwa (Version: 0.5.9-r16) we encountered cases where the proposed alignment position was outside of the reference sequence....