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GATK HP is missing high coverage variant sites
I am trying to use GATK HP ( v3.5 ) to call SNPs in amplicon seq data of a small genome of around 600bp. As shown in the attachment, the variants are... -
Best alternative for Nextera Indices for multiple amplicon resequencing experiment ?
Hi all,
after using the 96 plex double indexing kit (8 X 12 indices) of Illumina for 16s studies, we now apply this for other amplicon... -
ExoSap purified PCR product compatible for Illumina sequencing?
Hi All,
Does anyone have experience with purification of PCR amplicons using ExoSap Endonuclease treatment? I wonder if this procedure...
