A beginner user of sequencing work here.
I am going to run 100 samples on Miseq to look at the soil microbial communities (for bacteria, archaea...
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Forward and reverse reads overlap but don't align properly
I am working with Archaea F and R reads acquired with the 344f and 915r primers. Each read is 300bp long so there is overlap but it is only 29 bp. I want...
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What archaea apecific region/primers are best on MiSeq
I would like to create custom primers for MiSeq that will have a higher and more specific coverage of archaea. I have 2 questions pertaining to this:...