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What type of phred quality scores does samtools 1.3 expect
I am working with NG sequence data that was generated in 2012 using the Illumina HiSeq 1000. I have had some trouble getting samtools to accurately identify... -
Introducing Reformat, a fast read format converter
Reformat is a member of the BBMap/BBTools package. It is a multipurpose tool designed for converting reads or other nucleotide data between different... -
sequence and quality are inconsistent - modifying sam files to reflect differe ASCII
I ran into the "sequence and quality are inconsistent" error when trying to use samtools view -Sb on some bwa aln sam files. I read in a previous... -
Fastx giving error for Illumina data
Hi,
I'm trying to do some quality scoring/trimming on some Illumina, SE RNAseq data, but keep getting an unusual error message from Fastx....
