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Introductory NGS data analysis & variant calling workshops (online and in-person, early 2026)
For those looking for a structured introduction to NGS data analysis and DNA variant calling, we're running the same introductory workshop in two for...
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Problem with ONT sequencing pipeline. Probably in the Samtools part
Hi everyone. I have two sets of .fastq files from two ONT sequencing runs and one of them isn't working after generating .bam files.
The... -
Elevate Your NGS Skills: Join Our 2024 Online Course
Hello SeqAnswers Community,
Are you ready to take your Next-Generation Sequencing (NGS) skills to the next level? We're excited to announce... -
Elevate Your NGS Skills: Join Our 2024 Online Course
Hello SeqAnswers Community,
Are you ready to take your Next-Generation Sequencing (NGS) skills to the next level? We're excited to announce... -
Launched: Genozip 15 with co-compression of BAM and FASTQ
I am excited to announce the launch of our new version of Genozip - Genozip 15 - a genomic compressor for FASTQ, BAM, VCF and many other genomic formats....
Last edited by divon; 06-29-2023, 12:16 AM. -
STAR aligner : number of read
Hi,
Im' using star to map RNAseq sample in pair-end. But when I check the "..._Log.final.out" file it's mentioned for "number of... -
Help with subheads featureCounts
Hi All. I am new to bioinformatics, and am trying to run subReads featureCounts to analyze RNA seq data. I am using the code below, and am in the featureCounts...Last edited by lstarr; 07-24-2022, 05:26 PM. -
HTSeq-count: proper pairs in bam file
I have human RNA reads that I aligned against the human reference genome (GRCh 38) using BWA MEM and TopHat2. I now want to count the genes with HTSeq-count.... -
Coverage around genomic feature - large amount of data
Hi,
I have an alignment of human data (a small bam file with less than 1M reads), and would like to calculate and plot the coverage around... -
Compression of Ion Torrent BAM files
I am happy to announce that my Genozip tool now has an optimization feature specifically for Ion Torrent BAM files: --optimize-ZM optimizes the ZM field...Last edited by divon; 08-15-2021, 05:14 PM. -
Compressing Ion Torrent BAM files
Hi all,
I just thought it might be useful to announce here that my Genozip tool can very effectively compress IonTorrent BAM files with... -
Introducing Genozip - genomic file compressor
I would like to introduce a compression software package I developed: Genozip (www.genozip.com).
It is a tool with the primary objective... -
Combining multiple bams without losing library size information?
Hello all,
I have beeing searching the internet but I have not found what I need to know. Let's say that I have 3 or more bam files representing... -
Working with BAM files
Hi everybody!
This is my first thread in this forum.
Recently, I started an internship in a bioinformatics research group. Unfortunately,... -
Getting protein fasta from RNAseq BAM file (human) paying attention on repeats
Hi everybody,
I have several RNAseq BAM files (mapped with BWA and GATK IndelRealigner) of cancer patients of which I should compare if...
