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Help understanding directional libraries in bisulfite sequencing
Hi all, I know there are some other threads about this and I have read them, but for some reason I'm still struggling to understand the molecular biology... -
Bismark - extract genomic sequence from SAM / BAM format?
Hello all,
I am working with a bisulfite-converted library and I would like to extract the original genomic sequences for my reads (in... -
Bisulfite sequencing question
Hi all,
A (may be) stupid question about bisulfite sequencing: Do we in general expect CpGs to be equally methylated on both strands? For... -
Arioc: GPU-accelerated short-read alignment for BS-seq data
For folks who have a lot of BS-seq read data to align to a large reference genome, we have published an update to Arioc that aligns short bisulfite-treated... -
Subset for reads ending at a digest site
Hi, I have bisulfite converted reads (mapped as a bam file) and I would like to select only the reads that start and end at a Msp1 cut site (C^CGG) based... -
Postdoctoral Fellow (Epigenetics Liquid Biopsy Markers) - CSIRO, Sydney, Australia
Postdoctoral Fellowship on a project seeking to find and characterise circulating cell-free DNA biomarkers of traumatic brain injury.
- Based at
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Binary methylation states after bisulfite sequencing
Hi everyone. I have sequencing data (illumina Hiseq) from bisulfite treated samples (Arabidopsis). The bisulfite conversion of some selected regions was... -
BSMap bisulfite negative strand reads
Dear all,
I am using the BSMAP software to analyze bisulfite amplicon sequencing data. I am trying to calculate the bisulfite... -
New ssDNA-capable bisulfite sequencing library prep kit: Early Access Program
Swift Biosciences has developed what we believe is a best-in-class bisulfite sequencing library preparation kit. We have just opened the Early Access...Last edited by swiftbiosci; 05-14-2014, 10:52 AM. -
Target Enrichment from Bisulfite-treated DNA
New product for targeted sequencing from bisulfite-converted DNA
Press Release:
http://www.nimblegen.com/news/press_rel/news_20... -
SNP and indel calling in Bisulfite data
I am working on a bisulfite-treated sequence and I am interested in the SNP, indels, and methylated sites. I have used Bismark and bowtie2 to map the... -
Library prep question - ligation of P1 and P2 adapters
Hi there,
I'm trying to grasp how the nick translation works exactly after ligating the P1 and P2 adapters to A-tailed DNA fragments. Does... -
Bisulfite analysis with Illumina FastQ from different lanes
Hi everybody,
I'm a newbie in NGS bisulfite Methylation analysis, so first, apologies iof the question is so simple but I couldn't find... -
Methylation Analysis
What is the best option for methylation analysis at this point? We would like to analyze methylation in mouse liver samples and are considering all options:... -
PE bisulfite seq: could the EB solution inhibit the PCR rxn?
Hello everybody,
I am performing Paired End BISULFITE sequencing, with a modified Illumina protocol. I do the Covaris shearing, end repair, adenylation,...
