Hello,
I'm running some metagenomic data through blastn, and I'd like to have the output sorted by e-value.
The default...
22 results in 0.0029 seconds.
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Sort BlastN results by Evalue
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Needle and consensus seq?
Hi,
Our lab is currently sequencing a 1000pb gene fragment. They sequence foward and reverse strand, so they get the same strand sequenced...Last edited by Neuls; 12-16-2016, 12:09 AM. -
Problems with BLAST+ HSP
Dear Community
I have a very annoying problem
. In our laboratory we’re going to launch automated BLAST-searches. Therefore we’re...
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blastn help for primer alignment to custom db
Hello!
I am trying to find primer pairs that will amplify across 6 individuals of the same taxa. I have forward and reverse primers(<30bp... -
blastn perc_identity on matching region
Hi,
I am using blastn with the -perc_identity option. That way, blastn discards the alignments which do not fulfil a minimum % identities.... -
Time frame for BLASTN against NT
Hi Everyone,
I'm trying to find out a time frame I should expect for running blastn. I have about 50k sequences that I want to run against... -
bioedit vs blastn results
hello, I have blasted my contigs and obtained some hits with high percentage identities. I then retrieved the fasta files of those hits and computed sequence... -
How to run blastn against my data (and compare to ncbi nt library)?
I am new to blast and using bioinformatics tools on the command line. I recently have cleaned the data I was given (groomed, filtered, trimmed, and converted... -
Doing local blast. No alias or index file found for nucleotide database?
I am attempting to do a local blast nt. I have implemented the following procedure:- update_blast.pl nt
- tar zvpf nt.01.tar.gz command for each volume
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Installation of BLAST+ without root privileges
Hi,
I was trying to install BLAST+ as a dependency to an error correction tool SPRAI into a cluster server which I do not have root privileges,... -
indexing the nt database with makembindex to improve blastn (task megablast) runtime
I am trying to dust mask the NT database and then to index it using makembindex. However, when I run megablast on the indexed and dust masked nt database... -
blastn m0 -> m8 bug
Hi,all
Does anyone meet this question.
Blastn Error：
Error ：m=0, E-value = e-141，Gaps=3,... -
Blastn Results - analysis
Hi Everyone,
I have done some blasting on a draft genome that I assembled de novo(~300,000 contigs) and now I want to analyze the results.... -
Getting complete genome in BLASTn
When I use BLASTn to search for alignments, I get some alignments as well as some information about their location in the genome. I was wondering whether... -
blastn: restrict output to same length as query
Hi,
I would like to filter my blastn output to look at hits in their full length relative to the query, not just including the matching...
