8 results in 0.0022 seconds.
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Assembling haplotypes of a highly heterozygous gene cluster with canu
I am trying to assemble haplotypes for a peculiar region of the human genome that (1) has high heterozygosity, (2) has variation in presence or absence... -
SMRT Link 7.0 - HGAP
Has anyone gotten the HGAP assembler to work? It seems that it just barfs at different places constantly. Doesn't seem to be very robust at all.... -
Best practices for restarting Canu failed jobs?
Most of my Canu 1.8 jobs submitted in an HPC grid fail for various reasons. Is there a best practices document or web page that gives guidance for what... -
Assembling raw reads with Canu, but without correction
Hello friends,
I have raw ONP and PacBio reads for the human genome, ecoli, and yeast, and I want to test Canu's performance on them, without... -
Nanopolish 1D
I am working on 1D minion data, albacore 2.0 called, and attempting to use nanopolish on canu derived contigs.
I am trying to follow the github/readme... -
CANU-correct subreads individually VS batch
When my Pacbio sequencing was done I ended up with 18 subreads that each contained xxxx number of sequences (6,431,149 sequences in total for all 18 subreads).... -
Keep quality score when correcting with Canu assembler?
I am using Canu assembler to do a de novo assembly of PacBio data. I noticed that when I corrected my reads it produced a .fasta not a .fastq file, therefore... -
Canu error: 'failed to create the overlap store'
Trying to run the following command:
$ canu -trim -p PacBio10_trimmed -d PacBio10_trimmed genomeSize=200000 -pacbio-corrected /work/03868/lguzmanz/liz_trials/PacBio_correctedreads/corrected_Canu_pacbio10.correctedReads.fasta.gz...
