Dear all
I’ve sent a 8-indecies pooled sample for TruSeq Small RNA library illumina sequencing. Mention that I didn’t use kit and I used CATS...
19 results in 0.0029 seconds.
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cluster density problem in TruSeq Small RNA library sequencing
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Differential amplicon size effects on clustering across Illumina instruments
I would like to recommend to your attention a Bioarchive (pre-print) paper:
https://doi.org/10.1101/388108
entitled:... -
MiSeq still underclustered at 18 pM
Hello dear people,
We are doing some whole genome sequencing on a plant genome using the MiSeq with v3 reagents for 2x250 paired-end. Library size... -
NextSeq - Banded Clustering images
In September, I noticed that our NextSeq is producing a very odd banded clustering image. Specifically a there's a very bright band of clusters through... -
Obtaining cluster densities from a Hiseq2500 data set
Hi,
I'm making a wrapper for demultiplexing with bcl2fastq2 Conversion Software v2.17. Following demultiplexing I would like to collect various... -
mRNA library - final loading concentration for MiSeq?
Probably this has been covered before, but with the information scattered in numerous threads I'll ask again... What final loading concentrations are... -
Can we stop the run and re-generated the cluster on flow cell on MiSeq?
We are using the MiSeq machine (2*250, V2 kit) for a Truseq library sequencing. As we find the cluster density is low (only 239k) on the cycle 3, can... -
MiSeq run with low Q30 and high cluster PF
Hi all,
Could anybody give me an idea or though on the following?
I made a run with the MiSeq using the TrueSeq LT kit and... -
Low Cluster Densities in MiSEQ?? Possible explanations???
Dear All,
I have recently observed very low cluster densities when sequencing libraries on the MiSeq.
Briefly, I had loaded the Flow cell... -
Nextera XT Normalisation
Hi all,
I have been using the Nextera XT kit to do my library preps for MiSeq sequencing of fairly low complexity libraries and have been... -
Low cluster density of Truseq "high throughput" libraries - both DNA and RNA
Hi genomics folks,
Has anyone got any experience in clustering Illumina Truseq RNA 'HT' (high throughput) and Truseq DNA HT libraries?... -
MiSeq best result?
I am calling out all who think they have good MiSeq runs. Gaze at the perfection of this run and weep at the inadequacy of your wretched instruments:... -
Cluster density on a HiSeq2500
We are in the midst of our HiSeq2500 upgrade. First one for our intrepid FSE. Still some kinks in the process -- like one of the rear manifolds had an... -
Adventures in cluster density titration.
We have perennial issues with obtaining sane/consistent library concentrations prior to clustering. Those of you with a HiSeq will probably have this... -
MiSeq cluster generation problems
Hi all,
I'm running a library of synthetic amplicons (~175-250bp), and reading it out with custom primers on the MiSeq. I'm having a lot...
