Hello,
I have two different strains of the same bacteria, strain A and strain B. I assembled both of these using trinity de novo, and...
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Clustering/mapping genes across de novo assemblies
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sequences proceeding Illumina adapter
My question: If I have a a 24 nucleotide long sequence before the sequencing adapter would my DNA fragment still attach to the flow cells? Is there a...
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Cluster Radius Info in GAII/HiSeq Illumina platform
Hello GAII/HiSeq users,
Can someone tell me what is the typical radius of a cluster in Illumina GAII/HiSeq platform? I believe the total...
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Clustering of samples based on tetra nucleotide frequency
Hi All,
I have assembled several metagenomic samples of various sequencing depth. The contigs have been annotated with COG. Now I want...
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RNA-seq wheat-fungal timecourse
Hi there,
I'm finally posting rather than viewing as i'd appreciate some advice about my big experiment.
I am looking at the interaction...
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Loss of data in low-diversity libraries can be recovered by deferred cluster calling
It has been reported several times that low diversity at the start of Illumina sequencing libraries can lead to a large scale loss of data because the...