Hello,
I am giving a general talk to a room of undergraduates soon. For it I will draw a 5-D (3-D plus color and size of points) graph...
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Platform Comparison Table
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Platform Comparison Table
Hello,
I am giving a general talk to a room of undergraduates soon. For it I will draw a 5-D (3-D plus color and size of points) graph...
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Comparison of NGS technologies
Hi All
This is my first post!
I am trying to get my head around the various RNA-sequencing platforms so I thought of doing...
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multifactor analysis or pairwise comparison?
I can't find a simple answer to what's the difference between doing multifactor analysis or pairwise comparisons, example:
I have two factors...
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16S vs shotgun
Have people done detailed comparisons of bacterial communities profiled using amplicon (e.g. 16S) versus shotgun sequencing? I know HMP has done this...
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Oligo Synthesis Companies/Services
Hi everyone!
I've had one person tell me that IDT is the go-to company for oligo synthesis. Why is this?
I've gotten...
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MG-RAST - Efficient downloads?
Hello,
I want to download the hierarchical SEED Subsystems information for 30 metagenomes at MG-RAST. I've managed to obtain, through the API,...
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Comparing metagenomes produces with different sequencing technologies
Hi all,
I would like to compare the abundance of my gene(s) of interest across many published metagenomes. Typically we would normalize...
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Comparing two ChIP-seq libraries
Hello,
I am new to ChIP-seq data mining (by myself) and I am having a hard time comparing two ChIP-seq data sets. Could you tell me if...
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SEQC consortium manuscripts in NBT
A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequencing Quality Control Consortium
http://ww...
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Sampling for transcriptome analysis
Hi all,
I am going to do a transcriptome analysis of small scale S. cerevisiae fermentations using RNA-seq. I would like to get some feed-back...
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Viewing multiple BAM files in Artemis
Not a question - just found out how to do this and I don't think this is particularly well documented, so just posting this here to help other users....
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Is Sanger better than Next-Gen for Anything?
Do you think there are any applications for which Sanger sequencing (i.e. capillary electrophoresis, CE) still holds a competitive advantage over Next-Gen...