Hello everybody,
I'm running featureCounts (version 1.6.0) with an annotation in SAF format, this way :
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8 results in 0.0029 seconds.
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featureCounts : limits on multioverlap (option -O)
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Should discordant pairs be used for counting/DGE?
Hello everyone,
I am using the STAR aligner, which reports discordant pair alignments in a seperate BAM file. Using my workflow I want... -
creating GFF file from refernce fasta
HI,
I am working a flatworm for which the reference genome is not well characterized. I found the refernece trancriptome for my species... -
Pindel: Reference counting (Short Insertion)
I have a problem with counting sequences in reference to the Pindel software.
I am analyzing exome data (tumor vs. normal) and found a... -
counting wars ;) HTSeq vs RSEM
We have right now an internal lab discussion, a bit of classic "mapper wars" case. What is better, eg in the sense "closer to real biology":... -
count A/T/C/G/N in a aligned site
Hello,
I am wondering which tools I should use to count the A/T/C/G/N number/percentage in a given aligned site after alignment. Thanks... -
Counting distinct sequences in csfasta
I have an interesting problem that I'm not sure how to approach. I have a library of randomized short inserts (21 nt) that has been sequenced using the... -
DIY illumina adapters
Hi All:
Trying to determine whether I can get away with making my own adapters for the HiSEQ 2000.
I've got a library of small DNA (125nt)...
