I made bacterial RNA-seq libraries with ScriptSeq v2 and did a small sequencing test (MiSeq Nano, Single Read, 50 bp).
ScriptSeq v2 prepares...
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Seeing reads in both directions from a directional library?
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Directional Illumina read mapping for prokaryotic transcriptomes
Hello SeqAnswers!
I am looking for mapping software to analyze directional/strand-specific RNA-seq data from both paired-end and single-end... -
Bioinformatics protocols for differential RNAseq antisense expression
HI,
I am analyzing data coming form Illumina directional RNAseq procedure, in order to find antisense expression. I ran Tophat with the corresponding...Last edited by antgomo; 11-01-2012, 04:42 PM. -
Optimized Directional RNA-Seq Kits Incorporate Bias Reducing Technology
The patent pending NEXTflex™ Directional RNA-Seq Kits and Barcodes Primer Sets provide an easy, flexible and non-biased solution for generating directional... -
directional vs. non-directional sequencing
Hi,
Can someone please explain the difference between the directional and the non-directional sequencing methods?
I was... -
Illumina Directional RNA-Seq
Hi,
We recently got single end directional mRNA-Seq data obtained using the new Illumina protocol. I'm trying to map it to the genome using...Last edited by flobpf; 02-07-2011, 03:11 PM. -
Analysis of Directional mRNA-seq data / Illumina
We are developing a library preparation for directional mRNA-seq. All sequences will read 5' -> 3' direction. Our protocol isolates mRNA, fragments...
