Hi, this is my first post, so I hope I don't miss any details.
I'm trying to align RNA sequenced from the subcellular nucleoplasm fraction...
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aligning nucleoplasm fraction RNA
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High discordant alignments
I've set up a galaxy workflow for paired end first stranded RNAseq, and I've gotten some odd summary results from Tophat2 alignment. At least I think...
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Bam to Fastq on discordant mate pairs
Hi all!
I am trying to convert a BAM file from a paired-end alignment back to Fastq (Read1.fq and Read2.fq).
The BAM file...