Hi all,
I am running picard MarkDuplicates with REMOVE_DUPLICATES=TRUE.
From what I have read, if picard marks a primary alignment...
9 results in 0.0064 seconds.
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Picard MarkDuplicates and Secondary Reads
Last edited by Ham.m; 03-28-2017, 05:56 AM. -
ATAC-seq quality control
Hello,
I am analysing ATAC-seq data for the first time and am wondering what typical values are for:
Total number of reads... -
Counting Reads in BAM file per genomic position
Hello,
I am looking for a tool or script that counts the number of reads in a bam file by position, and takes into account only successfully...Last edited by C9r1y; 11-24-2015, 02:31 PM. -
ForeignMan started a topic Handling duplicates and other biases in Haloplex enriched targeted sequencing datain GeneralHandling duplicates and other biases in Haloplex enriched targeted sequencing data
Hello everyone,
we're starting to work with deep sequencing data of target regions that were enriched by Agilent's Haloplex kit (Illumina... -
Lib prep - Splitting PCR to reduce PCR bias
I want to reduce my high levels of duplicate reads (>50%) in my illumina HiSeq 100bp Paired end sequences.
The normal solutions aren't possible... -
Tools to remove duplicate reads
Hello, is there anyone aware of the difference in remove duplicate reads by using different tools. For example, the number of duplicate reads removed... -
High duplicate rates in exomes
Hello all,
We are currently carrying out analysis of a series of exomes prepared using the Illumina TruSeq exome selection kit, which targets... -
Duplicate Reads
Hi all,
I did a search of this forum and wasn't able to find a solution to my problem. Point me in a direction if this has already been... -
duplicate reads in enriched samples
Hi all,
I have some RNAseq data (enriched and non-enriched) generated on Illumina. I am seeing a lot of duplicate reads (as well as much higher...
