Hello,
I have an RNA-Seq experiment from insect antennae across different data points (0,2,4,6 and 8 days) with 7 replicates per data point...
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RNA-Seq Time series analysis in EdgeR
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Loss of relevant genes in NGS...
Dear SEQanswers community,
I have a quite fundamental concerns regarding using NGS to explore differentially expressed genes.
I...
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The Most Appropriate statistical Test
How to choose statistical test method appropriate to my experiment design?
I have following RNAseq samples. (I know 2 replicates is not enough...)...
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Examining possible extraneous variables in RNA-sequencing data
I have an RNA-sequencing dataset with 48 samples. I have a list of several possible extraneous variable values for each of the 48 samples. Some of these...
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Differential expression of miRNAs using edgeR
Hi,
I am working on elucidating pathologically relevant miRNAs responsible for disease progression and chemoresistance in chronic lymphocytic leukemia....
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How did the edgeR authors compute Figure 2 (genewise deviance statistics?)
**UPDATE**
I've migrated (aka copied) this question over to the biostars forum: https://www.biostars.org/p/244455/. Please look there for further...
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edgeR:prior.df value
Hello,
I'd like to know what prior.df stands for in edgeR algorithm and could this value be changed from default value to any other user...
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EdgeR and DEX
Hi to all I´m currently working with a pre-made R script to RNA-Seq using EdgeR, but I dont quite understand one of the outcome columns, please help....
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argument "dispersion" is missing, with no default
Hi!
I am trying to perform DEG analysis on two treatment groups using edgeR. I originally planned to keep the analysis similar to what...
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SuzuBell started a topic no slot of name "elementMetadata" for this object of class "SummarizedExperiment"in Generalno slot of name "elementMetadata" for this object of class "SummarizedExperiment"
I have a count table that was saved as an .rda file. I was always able to open the .rda file in R simply using load("myData.rda"). Recently,...
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edger and ccle
Hi all,
This is my first post here, so I am taking the opportunity to thank all the people who make this forum live.
...
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edgeR questions
Hello,
I have some questions regarding my RNAseq analysis process via edgeR. I have followed the official book LINK but sometimes it is...
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DEIVA: a web application for interactive visual analysis of differential gene express
Dear SEQanswers members,
we have developed a web app, DEIVA, for interactive visual analysis of differential gene expression results, such...
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Should I sequence un-replicated blocks in RNAseq?
Hi all,
I am designing a new experiment to sequence transcriptomes from wasps. I have 3 treatment "environments" and 4 replicate...