Hi,
Can anyone point me in the direction of a post or publication that lists how the droplet size for emPCR, the bead size and the well...
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Droplets, beads & wells
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problems with emPCR for GS Junior
Hi All,
I have an unusual problem with the consistency of emPCR kits that were received two months ago (emPCR oil and breaking kit (Lot:...
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454 Run Failure, emPCR and Libraries okay?
Greetings, forum. I am new here and I have searched for this problem in previous threads and have not found it. If this has already been discussed, I...
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low bead return from E120
Hello All, our last E120 only produced 700M beads (and not the expected ~2.2B). All of our templates looked good throughout the library process and we...
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sequencing primer annealing
Hi everybody
I am new here and would like to first say thank you for this great forum. I'm looking forward to being active in it.
I have...
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454 emPCR failure mystery
Hello,
I’ve had problems with a 454 FLX run and nobody (neither Roche/454 or my sequence provider) is able to work out what is going...
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SV Emulsion Breaking
I think this topic was addressed in another thread, but I can't find it in the sea of posts. So, I apologize for creating a new thread.
...
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Too low enrichment
High everybody
I am new to this community, it is great to have such a platform to ask questions!
My problem is the following:...