Hi All,
I am noticing evidence of chimeric reads coming from the ion proton, ie, the reads are half my organism of interest (lets say human) and...
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chimeric RNAseq reads on Ion Proton??
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ERCC ExFold vs. regular spike-in mix?
Does anyone know the practical difference between the Ambion ERCC RNA Spike-In Mix (Life Tech #4456740) and their ExFold mixes (#4456739)? The regular...
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DESeq analysis with ERCC RNA spike ins
Hi, I want to perform differential expression analyses of multiple RNA-seq samples using DESeq. We have included ERCC spike in controls and wish to use...
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Normalizing with ERCC spike-in
Hello everyone,
I have RNA-seq libraries prepared from 10 different stages of embryonic development (3 replicates per stage), with each...
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Pipeline + test datset for RNAseq with ERCC controls?
I'm putting together a pipeline to automate the process of analysing ERCC spike-in RNA controls in an RNAseq experiment. Just wondering if anyone is aware...Last edited by danwiththeplan; 03-10-2013, 02:03 PM.
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ERCC spike in controls for illumina?
Hello Seqers,
We have a illumina technician installing our HiSeq2000 as I write and I am trying to make some final improvements on my library constructions...
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Questions about ERCC RNA Spike-In Control
Hi everyone
We're hoping to start an RNASeq run on the Illumina HiSeq SQ with 50 bases paired-end sequencing in the next few weeks. I...