Trying to run the following command:
$ canu -trim -p PacBio10_trimmed -d PacBio10_trimmed genomeSize=200000 -pacbio-corrected /work/03868/lguzmanz/liz_trials/PacBio_correctedreads/corrected_Canu_pacbio10.correctedReads.fasta.gz...
9 results in 0.0021 seconds.
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Canu error: 'failed to create the overlap store'
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In-dels Illumina vs. PacBio
Hello,
I have a PacBio-generated closed complete bacteria genome. I am aligning Illumina reads of the same strain to it using breseq,... -
GATK UnifiedGenotyper with reducebam error
I am using GATK UnifiedGenotyper on multiple bam files for target enrichment sequencing. I get an error which although is a "user error", I... -
Tophat2.0.8b segment-junc err=1
Hi,
Last week I upgraded last version of Tophat; so now I'm using Tophat 2.0.8b with bowtie 1.0.0 (I need to align color space reads).
... -
How to fix Illumina FASTQ files with read length varies errors
How to fix FASTQ files with read length varies errors
0
Dear List,
I am a newbie in NGS analysis, I've got... -
Error in SeqClean
I'm using seqclean in an adapter trimming pipeline and have come across an error I can't track down. It seems to be environment specific as I can run... -
Bowtie Paired end 100% failed to align
Hello all,
I am trying to use Bowtie to align paired end sequencing data from solexa, but I am consistently getting
... -
Soap2 paired end mode
I have been trying to use the SOAP2 aligner in paired end mode with the following command:
...Code:./soap -a pair1.fastq -b pair2.fastq
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Soap Output File Error: Can't write
I am trying to use the soap2 aligner, but am getting the the error:
I am...Code:Output File Error: Can't write
